Human phospholipase C, epsilon 1 ELISA Kit | PLCE1 elisa kit

Human 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase epsilon-1, PLCE1 ELISA Kit

Cat. Num. AAA2602862

• Gene Names: PLCE1; PLCE; PPLC; NPHS3
• Reactivity: Human
• Synonyms: phospholipase C; epsilon 1; Human 1-phosphatidylinositol-4; 5-bisphosphate phosphodiesterase epsilon-1; PLCE1 ELISA Kit; PLCE1 elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.

• Samples: Human serum, plasma or cell culture supernatant and organizations
• Assay Type: Quantitative Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: 0.06 ng/ml
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLCE1 elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human PLCE1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 260166694
• NCBI GeneID: 51196
• NCBI Accession #: NP_001159451.1
• NCBI GenBank Nucleotide #: NM_001165979.2
• UniProt Accession #: Q9P212; Q1X6H8; Q5VWL4; Q5VWL5; Q9H9X8; Q9HBX6; Q9HC53; A6NGW0; A6NLA1; A7MBN7; A8K1D7; B9EIJ6
• Molecular Weight: 258,715 Da
• NCBI Official Full Name: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform 2
• NCBI Official Synonym Full Names: phospholipase C, epsilon 1
• NCBI Official Symbol: PLCE1
• NCBI Official Synonym Symbols: PLCE; PPLC; NPHS3
• NCBI Protein Information: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1; PLC-epsilon-1; pancreas-enriched phospholipase C; phosphoinositide phospholipase C-epsilon-1; phosphoinositide-specific phospholipase C epsilon-1
• UniProt Protein Name: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
• Protein Family: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
• UniProt Gene Name: PLCE1
• UniProt Synonym Gene Names: KIAA1516; PLCE; PPLC; PLC-epsilon-1
• UniProt Entry Name: PLCE1_HUMAN

NCBI Description

This gene encodes a phospholipase enzyme that catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate to generate two second messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). These second messengers subsequently regulate various processes affecting cell growth, differentiation, and gene expression. This enzyme is regulated by small monomeric GTPases of the Ras and Rho families and by heterotrimeric G proteins. In addition to its phospholipase C catalytic activity, this enzyme has an N-terminal domain with guanine nucleotide exchange (GEF) activity. Mutations in this gene cause early-onset nephrotic syndrome; characterized by proteinuria, edema, and diffuse mesangial sclerosis or focal and segmental glomerulosclerosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009]

Uniprot Description

PLCE1: a widely expressed bifunctional enzyme with phospholipase activity and Ras guanine-exchange factor (RasGEF) activity. Catalyzes the release of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) from 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate. Is an effector of heterotrimeric G protein activation. May play a role in cell survival, cell growth, actin organization and T-cell activation. Activated by the heterotrimeric G-protein subunits GNA12, GNA13 and GNB1-GNG2. Activated by HRAS, RAP1A, RHOA, RHOB, RHOC, RRAS and RRAS2. Activated by the G(s)-coupled GPCRs ADRB2, PTGER1 and CHRM3 through cyclic-AMP formation and RAP2B activation. Inhibited by G(i)-coupled GPCRs. Interacts with RHOA. Interacts with IQGAP1, HRAS, RAP1A, RAP2A, RAP2B and RRAS. Recruited to plasma membrane by activated HRAS and RAP2. Recruited to perinuclear membrane by activated RAP1A. Requires calcium for activiation. The Ras-associating domain 1 is degenerate and may not bind HRAS. The Ras-associating domain 2 mediates interaction with GTP-bound HRAS, RAP1A, RAP2A and RAP2B and recruitment of HRAS to the cell membrane. The Ras-GEF domain has a GEF activity towards HRAS and RAP1A. Mediates activation of the mitogen-activated protein kinase pathway. Defects in PLCE1 are the cause of nephrotic syndrome type 3 (NPHS3). Nephrotic syndrome, a malfunction of the kidney glomerular filter, leads to proteinuria, hypoalbuminemia, edema. Overexpressed during heart failure. A GWAS study identified an association between esophageal squamous cell carcinoma and a common DNA variant in the PLCE1 gene in East Asians. Two isoforms of the human protein are produced by alternative splicing. Isoform 1 is broadly expressed and only absent in peripheral blood leukocytes. Isoform 2 is specifically expressed in placenta, lung and spleen. Isoform 1 and isoform 2 associate with Golgi membranes.

Protein type: EC 3.1.4.11; Phospholipase; GAPs, Ras; Carbohydrate Metabolism - inositol phosphate; GAPs

Chromosomal Location of Human Ortholog: 10q23

Cellular Component: Golgi membrane; cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; Ras GTPase binding; enzyme binding; guanyl-nucleotide exchange factor activity; receptor signaling protein activity; phosphoinositide phospholipase C activity; phospholipase C activity

Biological Process: epidermal growth factor receptor signaling pathway; regulation of Ras protein signal transduction; regulation of smooth muscle contraction; inositol phosphate-mediated signaling; inositol phosphate metabolic process; activation of MAPK activity; diacylglycerol biosynthetic process; heart development; calcium-mediated signaling; cytoskeleton organization and biogenesis; glomerulus development; cell proliferation; elevation of cytosolic calcium ion concentration; phospholipid metabolic process; Ras protein signal transduction; protein kinase C activation; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of cell growth; regulation of protein kinase activity; regulation of G-protein coupled receptor protein signaling pathway; lipid catabolic process; positive regulation of GTPase activity

Disease: Nephrotic Syndrome, Type 3