Western Blot (WB)
(Western blot analysis of TMEM16B in human small intestine tissue lysate with TMEM16B antibody at (A) 1 and (B) 2 μg/mL.)
Rabbit TMEM16B Polyclonal Antibody | anti-ANO2 antibody
TMEM16B Antibody
Gene Names
ANO2; C12orf3; TMEM16B
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
TMEM16B Antibody is affinity chromatography purified via peptide column.
Synonyms
TMEM16B; Polyclonal Antibody; TMEM16B Antibody; C12orf3; Anoctamin-2; Transmembrane protein 16B; anoctamin 2; anti-ANO2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
TMEM16B Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
999
Applicable Applications for anti-ANO2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
TMEM16B antibody can be used for detection of TMEM16B by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
TMEM16B antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human TMEM16B.
Buffer
TMEM16B Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
TMEM16B antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of TMEM16B in human small intestine tissue lysate with TMEM16B antibody at (A) 1 and (B) 2 μg/mL.)
Western Blot (WB)
(Western blot analysis of TMEM16B in human small intestine tissue lysate with TMEM16B antibody at (A) 1 and (B) 2 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of TMEM16B in human brain tissue with FRMPD4 antibody at 5 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of TMEM16B in human brain tissue with FRMPD4 antibody at 5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of TMEM16B in Human Brain cells with TMEM16B antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of TMEM16B in Human Brain cells with TMEM16B antibody at 20 μg/mL.)
Related Product Information for anti-ANO2 antibody
TMEM16B Antibody: Calcium-activated chloride channels (CaCC) are present in many cell types and mediate physiological functions such as epithelial secretion, sensory signal transduction, and smooth muscle contraction. Subunits of these CaCC's include the transmembrane proteins TMEM16A and TMEM16B. TMEM16B is predicted to have eight transmembrane domains with both the amino and carboxy termini in the cytoplasm and is expressed in several tissues including olfactory sensory neurons as well as photoreceptors in mammalian retina. Like TMEM16A, TMEM16B is thought to form at least part of CaCC's but has different biophysical characteristics such as voltage dependence and unitary conductance.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
113,616 Da
NCBI Official Synonym Full Names
anoctamin 2, calcium activated chloride channel
NCBI Official Symbol
ANO2
NCBI Official Synonym Symbols
C12orf3; TMEM16B
NCBI Protein Information
anoctamin-2
UniProt Protein Name
Anoctamin-2
Protein Family
UniProt Gene Name
ANO2
UniProt Synonym Gene Names
C12orf3; TMEM16B
UniProt Entry Name
ANO2_HUMAN
NCBI Description
ANO2 belongs to a family of calcium-activated chloride channels (CaCCs) (reviewed by Hartzell et al., 2009 [PubMed 19015192]).[supplied by OMIM, Jan 2011]
Uniprot Description
ANO2: Acts as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. May mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina. Belongs to the anoctamin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Transporter, ion channel; Membrane protein, integral; Membrane protein, multi-pass; Transporter
Chromosomal Location of Human Ortholog: 12p13.3
Cellular Component: nucleoplasm; nonmotile primary cilium; plasma membrane
Molecular Function: protein homodimerization activity; intracellular calcium activated chloride channel activity; protein heterodimerization activity
Biological Process: transmembrane transport
Research Articles on ANO2
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Product Notes
The ANO2 ano2 (Catalog #AAA150565) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TMEM16B Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TMEM16B can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). TMEM16B antibody can be used for detection of TMEM16B by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the ANO2 ano2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TMEM16B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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