Western Blot (WB)
(Western blot analysis of TMEM16A in A549 cell lysate with TMEM16A antibody at 1 μg/mL.)
Rabbit anti-Human, Mouse TMEM16A Polyclonal Antibody | anti-ANO1 antibody
TMEM16A Antibody
Gene Names
ANO1; DOG1; TAOS2; ORAOV2; TMEM16A
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
TMEM16A Antibody is affinity chromatography purified via peptide column.
Synonyms
TMEM16A; Polyclonal Antibody; TMEM16A Antibody; DOG1; TAOS2; ORAOV2; Anoctamin-1; Discovered on gastrointestinal stromal tumors protein 1; anoctamin 1; calcium activated chloride channel; anti-ANO1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
TMEM16A Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
986
Applicable Applications for anti-ANO1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
TMEM16A antibody can be used for detection of TMEM16A by Western blot at 1 mug/mL.
Conjugate
Unconjugated
Immunogen
TMEM16A antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human TMEM16A.
Buffer
TMEM16A Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
TMEM16A antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of TMEM16A in A549 cell lysate with TMEM16A antibody at 1 μg/mL.)
Western Blot (WB)
(Western blot analysis of TMEM16A in A549 cell lysate with TMEM16A antibody at 1 μg/mL.)
Related Product Information for anti-ANO1 antibody
TMEM16A Antibody: Calcium-activated chloride channels (CaCC) are present in many cell types and mediate physiological functions such as epithelial secretion, sensory signal transduction, and smooth muscle contraction. Subunits of these CaCC's include the transmembrane proteins TMEM16A and TMEM16B. TMEM16A is expressed in epithelial cells of the kidney and lung, pancreas, and sensory neurons and its mRNA is seen in foregut, airway epithelia, and tracheal smooth muscle; mice lacking TMEM16A fail to survive past ten days and show aerophagia and little weight gain. TMEM16A is also overexpressed or amplified in multiple cancers associated with poor survival such as oral cancers and gastrointestinal stromal tumors, suggesting the development of CaCC modulators may be a viable therapeutic strategy.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
74,369 Da
NCBI Official Full Name
anoctamin-1
NCBI Official Synonym Full Names
anoctamin 1, calcium activated chloride channel
NCBI Official Symbol
ANO1
NCBI Official Synonym Symbols
DOG1; TAOS2; ORAOV2; TMEM16A
NCBI Protein Information
anoctamin-1; Ca2+-activated Cl- channel; oral cancer overexpressed 2; tumor-amplified and overexpressed sequence 2; discovered on gastrointestinal stromal tumors protein 1; transmembrane protein 16A (eight membrane-spanning domains)
UniProt Protein Name
Anoctamin-1
Protein Family
UniProt Gene Name
ANO1
UniProt Synonym Gene Names
DOG1; ORAOV2; TAOS2; TMEM16A
UniProt Entry Name
ANO1_HUMAN
Uniprot Description
ANO1: Acts as a calcium-activated chloride channel. Required for normal tracheal development. Belongs to the anoctamin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Transporter; Membrane protein, integral; Transporter, ion channel; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 11q13.3
Cellular Component: apical plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane
Molecular Function: protein binding; protein homodimerization activity; intracellular calcium activated chloride channel activity; protein heterodimerization activity; calcium activated cation channel activity
Biological Process: regulation of membrane potential; multicellular organismal development; chloride transport; transmembrane transport; cation transport
Research Articles on ANO1
Similar Products
Product Notes
The ANO1 ano1 (Catalog #AAA151482) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TMEM16A Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TMEM16A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). TMEM16A antibody can be used for detection of TMEM16A by Western blot at 1 mug/mL. Researchers should empirically determine the suitability of the ANO1 ano1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TMEM16A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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