Western Blot (WB)
(Western blot analysis of SLC1A7 in C2C12 cell lysate with SLC1A7 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
Rabbit anti-Human, Mouse SLC1A7 Polyclonal Antibody | anti-SLC1A7 antibody
SLC1A7 Antibody
Gene Names
SLC1A7; AAAT; EAAT5
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
SLC1A7 antibody is affinity chromatography purified via peptide column.
Synonyms
SLC1A7; Polyclonal Antibody; SLC1A7 Antibody; Solute carrier family 1 (glutamate transporter) member 7; AAAT; excitatory amino acid transporter 5; EAAT5; solute carrier family 1 (glutamate transporter); member 7; anti-SLC1A7 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
SLC1A7 antibody is human and mouse reactive. Multiple isoforms of SLC1A7 are known to exist.
Purity/Purification
SLC1A7 antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
619
Applicable Applications for anti-SLC1A7 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
SLC1A7 antibody can be used for detection of SLC1A7 by Western blot at 1 - 2 mug/ml. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
SLC1A7 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human SLC1A7.
Buffer
SLC1A7 antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
SLC1A7 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
Western Blot (WB)
(Western blot analysis of SLC1A7 in C2C12 cell lysate with SLC1A7 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
Western Blot (WB)
(Western blot analysis of SLC1A7 in C2C12 cell lysate with SLC1A7 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
Immunohistochemistry (IHC)
(Immunohistochemistry of SLC1A7 in mouse kidney tissue with SLC1A7 antibody at 5 μg/ml.)
Immunohistochemistry (IHC)
(Immunohistochemistry of SLC1A7 in mouse kidney tissue with SLC1A7 antibody at 5 μg/ml.)
Immunofluorescence (IF)
(Immunofluorescence of SLC1A7 in mouse kidney tissue with SLC1A7 antibody at 20 μg/ml.)
Immunofluorescence (IF)
(Immunofluorescence of SLC1A7 in mouse kidney tissue with SLC1A7 antibody at 20 μg/ml.)
Related Product Information for anti-SLC1A7 antibody
The solute carrier family 1 member 7 (SLC1A7) protein was initially identified as a glutamate transporter coupled to a chloride conductance that is expressed primarily in the retina (1). More recent evidence indicates that is it widely expressed in peripheral tissues, also (2). Experiments have shown that SLC1A7 functions as a low-affinity/low-capacity glutamate transport, with an anion channel optimized for anion conductance in the negative voltage range (3). In the retina, SLC1A7 co-localizes with the serum and glucocorticoid dependent kinase SGK1, and its cell surface expression and activity is regulated SGK1 and its isoform SGK3 (4).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 17; 52; 62; 68 kDa
Observed: 52 kDa
Observed: 52 kDa
NCBI Official Full Name
excitatory amino acid transporter 5 isoform 1
NCBI Official Synonym Full Names
solute carrier family 1 (glutamate transporter), member 7
NCBI Official Symbol
SLC1A7
NCBI Official Synonym Symbols
AAAT; EAAT5
NCBI Protein Information
excitatory amino acid transporter 5; retinal glutamate transporter; excitatory amino acid transporter 5 (retinal glutamate transporter)
UniProt Protein Name
Excitatory amino acid transporter 5
Protein Family
UniProt Gene Name
SLC1A7
UniProt Synonym Gene Names
EAAT5
UniProt Entry Name
EAA5_HUMAN
Uniprot Description
SLC1A7: Transports L-glutamate; the L-glutamate uptake is sodium- and voltage-dependent and chloride-independent. Its associated chloride conductance may participate in visual processing. Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A7 subfamily.
Protein type: Membrane protein, integral; Transporter; Transporter, SLC family; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 1p32.3
Cellular Component: integral to membrane; plasma membrane
Molecular Function: sodium:dicarboxylate symporter activity; L-glutamate transmembrane transporter activity
Biological Process: dicarboxylic acid transport; ion transport; transmembrane transport
Research Articles on SLC1A7
Similar Products
Product Notes
The SLC1A7 slc1a7 (Catalog #AAA153404) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SLC1A7 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SLC1A7 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). SLC1A7 antibody can be used for detection of SLC1A7 by Western blot at 1 - 2 mug/ml. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the SLC1A7 slc1a7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLC1A7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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