Mouse anti-Human Brachyury Monoclonal Antibody | anti-TBXT antibody

Anti-Brachyury Antibody, Mouse Monoclonal

Cat. Num. AAA8100252

• Gene Names: T; TFT; SAVA
• Reactivity: Human
• Applications: Immunocytochemistry, Immunofluorescence
• Purity: Protein A
• Synonyms: Brachyury; Monoclonal Antibody; Anti-Brachyury Antibody; Mouse Monoclonal; Brachyury/TBXT Antibody; Mouse MAb; T-box transcription factor T; Anti-SAVA Antibody; Anti-TFT Antibody; anti-TBXT antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone Number: 07
• Specificity: Human Brachyury/TBXT
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-TBXT antibody: Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: ICC/IF: 1:20-1:100
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human Brachyury/TBXT
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the C-terminus of the Human Brachyury/TBXT. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence staining of TBXT in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-Human T monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to Nucleus.)

Related Product Information for anti-TBXT antibody

Brachyury is encoded by the TBXT gene. TBXT plays in the Cnidaria, appears to be in defining the blastopore. Brachyury is an important factor in promoting the epithelial-mesenchymal transition (EMT). Cells that over-express brachyury have down-regulated expression of the adhesion molecule E-cadherin, which allows them to undergo EMT. This process is at least partially mediated by the transcription factors AKT and Snail. Brachyury is overexpressed in a number of tumor types.

NCBI and Uniprot Product Information

• NCBI GI #: 394953947
• NCBI GeneID: 6862
• NCBI Accession #: NP_001257413.1
• NCBI GenBank Nucleotide #: NM_001270484.1
• UniProt Accession #: O15178; Q4KMP4; E7ERD6
• Molecular Weight: 41,078 Da
• NCBI Official Full Name: brachyury protein isoform 2
• NCBI Official Synonym Full Names: T brachyury transcription factor
• NCBI Official Symbol: T
• NCBI Official Synonym Symbols: TFT; SAVA
• NCBI Protein Information: brachyury protein; T brachyury homolog; T, brachyury homolog; protein T
• UniProt Protein Name: Brachyury protein
• Protein Family: Brachyury protein
• UniProt Gene Name: T
• UniProt Entry Name: BRAC_HUMAN

NCBI Description

The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

Brachyury: Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site. Genetic variations in T are associated with susceptibility to neural tube defects (NTD). NTD are common congenital malformations. Spina bifida, which results from malformations in the caudal region of the neural tube, is compatible with life but associated with significant morbidity, including lower limb paralysis. T is involved in susceptibility to the development of chordoma (CHDM). Chordomas are rare, clinically malignant tumors derived from notochordal remnants. They occur along the length of the spinal axis, predominantly in the sphenooccipital, vertebral and sacrococcygeal regions. They are characterized by slow growth, local destruction of bone, extension into adjacent soft tissues and rarely, distant metastatic spread. Susceptibility to development of chordomas is due to a T gene duplication.

Protein type: Motility/polarity/chemotaxis; DNA-binding; Cell cycle regulation; Transcription factor

Chromosomal Location of Human Ortholog: 6q27

Cellular Component: cytoplasm; nuclear chromatin; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; heart morphogenesis; somitogenesis; neural plate morphogenesis; embryonic skeletal development; mesoderm migration; notochord formation; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; signal transduction; BMP signaling pathway; determination of anterior/posterior axis, embryo; positive regulation of cell proliferation; neural tube closure; penetration of zona pellucida; mesoderm development; vasculogenesis

Disease: Neural Tube Defects; Sacral Agenesis With Vertebral Anomalies