Rabbit TLR2 Polyclonal Antibody | anti-TLR2 antibody

TLR2 Antibody

Cat. Num. AAA841226

• Gene Names: Tlr2; Ly105
• Reactivity: Human, Mouse, Rat, Monkey
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: TLR2; Polyclonal Antibody; TLR2 Antibody; Anti-TLR2; CD282; TIL4; Toll-like receptor 2; anti-TLR2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Monkey
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 784

• Applicable Applications for anti-TLR2 antibody: Western Blot (WB)
• Application Notes: Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~86 kDa TLR2 in Jurkat cell lysate. Reactivity to other species has not been tested. Blocking peptide is available separately.
• Immunogen: Synthetic peptide surrounding amino acid 753 of mouse TLR2
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-TLR2 antibody

Background: The Toll-like receptor (TLR) family in mammalian comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Up-to-date, ten TLRs (TLR1-10) have been described. TLR2 is differentially expressed in human cells. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells. CD3+ T-cells and CD56+ NK cells did not express TLR2. The expression of TLR2 on different cell types is regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulate TLR2, whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.

NCBI and Uniprot Product Information

• NCBI GI #: 21955346
• NCBI GeneID: 24088
• Molecular Weight: 89,449 Da
• NCBI Official Full Name: Toll-like receptor 2
• NCBI Official Synonym Full Names: toll-like receptor 2
• NCBI Official Symbol: Tlr2
• NCBI Official Synonym Symbols: Ly105
• NCBI Protein Information: toll-like receptor 2
• UniProt Protein Name: Toll-like receptor 2
• Protein Family: Toll-like receptor
• UniProt Gene Name: Tlr2
• UniProt Entry Name: TLR2_MOUSE

Uniprot Description

TLR2: Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage- activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6. Interacts with LY96, TLR1 and TLR6 (via extracellular domain). Binds MYD88 (via TIR domain). Interacts with TICAM1. Ligand binding induces the formation of a heterodimer with TLR1. Interacts with CNPY3. Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues. Belongs to the Toll-like receptor family.

Protein type: Cell surface; Membrane protein, integral; Receptor, misc.; Apoptosis; Motility/polarity/chemotaxis

Cellular Component: cell projection; cell surface; membrane; cytoplasm; integral to membrane; plasma membrane; cytoplasmic vesicle; external side of plasma membrane

Molecular Function: peptidoglycan binding; protein binding; transmembrane receptor activity; protein heterodimerization activity; triacylated lipoprotein binding; receptor activity; pattern recognition receptor activity; diacylated lipoprotein binding

Biological Process: regulation of cytokine secretion; positive regulation of nitric oxide biosynthetic process; positive regulation of interleukin-12 production; positive regulation of leukocyte migration; microglial cell activation; positive regulation of NF-kappaB import into nucleus; leukotriene metabolic process; detection of triacylated bacterial lipoprotein; response to molecule of fungal origin; signal transduction; positive regulation of interleukin-18 production; nitric oxide metabolic process; positive regulation of interleukin-10 production; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; negative regulation of cell proliferation; response to peptidoglycan; response to molecule of bacterial origin; positive regulation of interferon-beta production; response to bacterial lipoprotein; positive regulation of oligodendrocyte differentiation; inflammatory response; positive regulation of Wnt receptor signaling pathway; positive regulation of tumor necrosis factor biosynthetic process; negative regulation of interleukin-12 production; immune system process; detection of diacylated bacterial lipoprotein; cell surface pattern recognition receptor signaling pathway; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of chemokine production; positive regulation of toll-like receptor signaling pathway; toll-like receptor 2 signaling pathway; induction by symbiont of defense-related host nitric oxide production; MyD88-dependent toll-like receptor signaling pathway; defense response to Gram-positive bacterium; negative regulation of interleukin-17 production; myelin formation in the central nervous system; response to bacterium; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; immune response; I-kappaB phosphorylation; chloramphenicol transport; positive regulation of cytokine secretion; positive regulation of inflammatory response; positive regulation of nitric-oxide synthase biosynthetic process