Rabbit TLR5 Polyclonal Antibody | anti-TLR5 antibody

TLR5 Antibody

Cat. Num. AAA840951

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: TLR5; Polyclonal Antibody; TLR5 Antibody; Anti-TLR5; FLJ10052; MGC126430; MGC126431; TLR 5; anti-TLR5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) affinity purified rabbit anti-TLR5 polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 873

• Applicable Applications for anti-TLR5 antibody: Western Blot (WB)
• Application Notes: Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. Other applications have not been determined. The antibody recognizes ~97 kDa TLR5 in sample from human, mouse and rat origins. Reactivity to other species has not been tested. Blocking peptide is available separately.
• Immunogen: Synthetic peptide surrounding amino acid 829 of human TLR5
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-TLR5 antibody

Background: The Toll-like receptor (TLR) family of proteins are characterized by a highly conserved Toll homology (TH) domain, which is essential for Toll-induced signal transduction. TLR1, as well as the other TLR family members, are type I transmembrane receptors that characteristically contain an extracellular domain consisting of several leucine-rich regions along with a single cytoplasmic Toll/IL-1R-like domain. TLR2 and TLR4 are activated in response to lipopolysacchride (LPS) stimulation, which results in the activation and translocation of NFkB and smuggests that these receptors are involved in mediating inflammatory responses. TLR6 is highly homologous to TLR1, and like other members of TLR family, it induces NFkB signaling upon activation. Expression of TLR receptors is highest in peripheral blood leukocytes, macrophages, and monocytes.

NCBI and Uniprot Product Information

• NCBI GI #: 124248590
• NCBI GeneID: 53791
• NCBI Accession #: NP_058624
• NCBI GenBank Nucleotide #: NM_016928.2
• Molecular Weight: 97,627 Da
• NCBI Official Full Name: toll-like receptor 5
• NCBI Official Synonym Full Names: toll-like receptor 5
• NCBI Official Symbol: Tlr5
• NCBI Protein Information: toll-like receptor 5
• UniProt Protein Name: Toll-like receptor 5
• Protein Family: Toll-like receptor
• UniProt Gene Name: Tlr5
• UniProt Entry Name: TLR5_MOUSE

Uniprot Description

TLR5: Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Genetic variation in TLR5 is associated with resistance to systemic lupus erythematosus type 1 (SLEB1). Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with a complex genetic basis. SLE is an inflammatory, and often febrile multisystemic disorder of connective tissue characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Belongs to the Toll-like receptor family.

Protein type: Membrane protein, integral; Receptor, misc.

Cellular Component: membrane; integral to membrane

Molecular Function: protein binding; interleukin-1 receptor binding; transmembrane receptor activity

Biological Process: toll-like receptor 5 signaling pathway; positive regulation of interleukin-8 production; positive regulation of cytokine production; MyD88-dependent toll-like receptor signaling pathway; regulation of cytokine secretion; positive regulation of nitric oxide biosynthetic process; immune system process; defense response to bacterium; innate immune response; signal transduction; inflammatory response; positive regulation of toll-like receptor signaling pathway