TLR2 recombinant protein

Recombinant Mouse TLR2 Protein (His tag)

Cat. Num. AAA2546946

• Gene Names: Tlr2; Ly105
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: TLR2; Recombinant Mouse TLR2 Protein (His tag); TLR2 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly
• Sequence Length: 784

• Application Notes: The recombinant mouse TLR2 consists of 573 amino acids and has a calculated molecular mass of 64.8 kDa. The recombinant protein migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Gln 25
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for TLR2 recombinant protein

Background: TLR2, also known as CD282, is a member of the Toll-like receptor (TLR) family. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They play a fundamental role in pathogen recognition and activation of innate immunity. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. TLR2 contains 14 LRR (leucine-rich) repeats and 1 TIR domain. TLR2 gene is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. CD282 cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. It also cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. CD282 acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. It may also promote apoptosis in response to lipoproteins.

Description: A DNA sequence encoding the mouse TLR2 (Q9QUN7) (Met1-Gln587) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 158749638
• NCBI GeneID: 24088
• NCBI Accession #: NP_036035.3
• NCBI GenBank Nucleotide #: NM_011905.3
• UniProt Accession #: Q9QUN7; Q3U400; Q9DBC4
• Molecular Weight: 89,449 Da
• NCBI Official Full Name: toll-like receptor 2
• NCBI Official Synonym Full Names: toll-like receptor 2
• NCBI Official Symbol: Tlr2
• NCBI Official Synonym Symbols: Ly105
• NCBI Protein Information: toll-like receptor 2
• UniProt Protein Name: Toll-like receptor 2
• Protein Family: Toll-like receptor
• UniProt Gene Name: Tlr2

Uniprot Description

TLR2: Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage- activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6. Interacts with LY96, TLR1 and TLR6 (via extracellular domain). Binds MYD88 (via TIR domain). Interacts with TICAM1. Ligand binding induces the formation of a heterodimer with TLR1. Interacts with CNPY3. Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues. Belongs to the Toll-like receptor family.

Protein type: Apoptosis; Cell surface; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.

Chromosomal Location of Human Ortholog: 3|3 E3

Cellular Component: cell projection; cell surface; cytoplasm; external side of plasma membrane; Golgi apparatus; intrinsic to plasma membrane; lipid raft; plasma membrane

Molecular Function: diacylated lipoprotein binding; lipopolysaccharide binding; pattern recognition receptor activity; peptidoglycan binding; protein binding; protein heterodimerization activity; receptor activity; triacylated lipoprotein binding

Biological Process: activation of NF-kappaB transcription factor; cell surface pattern recognition receptor signaling pathway; chloramphenicol transport; cytokine secretion during immune response; defense response to Gram-positive bacterium; detection of diacylated bacterial lipoprotein; detection of triacylated bacterial lipoprotein; I-kappaB phosphorylation; induction by symbiont of defense-related host nitric oxide production; inflammatory response; innate immune response; interleukin-10 production; leukotriene metabolic process; microglial cell activation; MyD88-dependent toll-like receptor signaling pathway; myelin formation in the central nervous system; negative regulation of cell proliferation; negative regulation of interleukin-12 production; negative regulation of interleukin-17 production; nitric oxide metabolic process; positive regulation of chemokine production; positive regulation of cytokine secretion; positive regulation of interferon-beta production; positive regulation of interleukin-10 production; positive regulation of interleukin-12 production; positive regulation of interleukin-18 production; positive regulation of interleukin-6 production; positive regulation of interleukin-8 production; positive regulation of leukocyte migration; positive regulation of NF-kappaB import into nucleus; positive regulation of nitric oxide biosynthetic process; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of oligodendrocyte differentiation; positive regulation of toll-like receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tumor necrosis factor biosynthetic process; positive regulation of tumor necrosis factor production; positive regulation of Wnt receptor signaling pathway; regulation of dendritic cell cytokine production; response to bacterial lipoprotein; response to bacterium; response to molecule of fungal origin; response to peptidoglycan; toll-like receptor signaling pathway