Rabbit Stat1 Polyclonal Antibody | anti-Stat1 antibody

Stat1 Antibody

Cat. Num. AAA840203

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: Stat1; Polyclonal Antibody; Stat1 Antibody; signal transducer and activator of transcription 1; anti-Stat1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 749

• Applicable Applications for anti-Stat1 antibody: Western Blot (WB)
• Application Notes: Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody recognizes both alpha (91 kDa) and beta (84 kDa) subunits of STAT1 in samples from human, mouse, and rat origins.
• Immunogen: Synthetic peptide surrounding amino acid 672 of rat Stat1
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-Stat1 antibody

Background: STATs (signal transducers and activators of transcription) are a family of cytoplamic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signalling peptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6). Stat1, Stat3, Stat5a and Stat5b have wide activation profiles. Stat1 is activated by many different ligands including IFN family (IFN-a, IFN-b, IFN-r, and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF-1), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). Stat1 has two forms, the 91 kDa STAT1alpha and the 84 kDa STAT1beta, which are coded by the same gene with splicing variant.

NCBI and Uniprot Product Information

• NCBI GI #: 6636498
• Molecular Weight: 87,234 Da
• NCBI Official Full Name: signal transducer and activator of transcription 1
• UniProt Protein Name: Signal transducer and activator of transcription
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: Stat1
• UniProt Entry Name: Q9QXK0_RAT

NCBI Description

component of the IFN-gamma receptor signaling pathway and other signaling pathways; plays a role in development of cytokine resistance [RGD, Feb 2006]

Uniprot Description

STAT1: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of certain receptor tyrosine kinases, GPCRs, and receptors for various interleukins and interferons. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: Transcription factor; DNA-binding

Cellular Component: nucleoplasm; axon; dendrite; nuclear chromatin; cytoplasm; nucleolus; nucleus

Molecular Function: identical protein binding; signal transducer activity; protein homodimerization activity; enzyme binding; DNA binding; protein phosphatase 2A binding; sequence-specific DNA binding; double-stranded DNA binding; tumor necrosis factor receptor binding; transcription factor activity; CCR5 chemokine receptor binding

Biological Process: transcription from RNA polymerase II promoter; response to peptide hormone stimulus; response to cAMP; blood circulation; positive regulation of transcription, DNA-dependent; positive regulation of smooth muscle cell proliferation; response to lipopolysaccharide; response to organic cyclic substance; response to exogenous dsRNA; tumor necrosis factor-mediated signaling pathway; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; negative regulation of viral protein levels in host cell; lipopolysaccharide-mediated signaling pathway; response to nutrient; caspase activation; response to drug; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; JAK-STAT cascade; regulation of transcription from RNA polymerase II promoter; negative regulation of angiogenesis; response to hydrogen peroxide; response to bacterium; cellular response to insulin stimulus; response to mechanical stimulus; response to cytokine stimulus; negative regulation of endothelial cell proliferation; endothelial cell migration; positive regulation of transcription from RNA polymerase II promoter