Rabbit anti-Human TIMP-4 (Tissue Inhibitor of Metalloproteinases 4) Polyclonal Antibody | anti-TIMP-4 antibody

Anti-TIMP-4 (Tissue Inhibitor of Metalloproteinases 4), Loop 2 Antibody

Cat. Num. AAA4157383

• Reactivity: Human
• Applications: Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: TIMP-4 (Tissue Inhibitor of Metalloproteinases 4); Polyclonal Antibody; Anti-TIMP-4 (Tissue Inhibitor of Metalloproteinases 4); Loop 2 Antibody; anti-TIMP-4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: The antibody recognises TIMP4. It does not cross react with the other TIMP family members (TIMP1, TIMP2, TIMP3).
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)

• Applicable Applications for anti-TIMP-4 antibody: Western Blot (WB)
• Application Notes: Anti-TIMP-4 works well for Western Blotting applications, and when used against the reduced protein identifies bands at 23 and 29 Kd, representing the unglycosylated and glycosylated forms respectively of TIMP-4. TIMP-4 has been reported in heart tissues, and is produced by some fibroblasts in cell culture conditions. Treatment of cells with the phorbol ester TPA stimulates production of TIMP-4 in some cell types, but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples.
• Immunogen: Synthetic peptide based on loop 2 of human TIMP4
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-TIMP-4 antibody

Description: The antibody is a Rabbit antibody to the Tissue Inhibitor of Metalloproteinases-4, TIMP-4, made with a synthetic peptide based on loop #2 of the Human sequence. The antibody binds to TIMP-4, but does not cross react with the other TIMP family members (TIMP-1, TIMP-2, TIMP-3). The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of Sodium Azide as preservative and 50% glycerol as cryoprotectant.

Relevance: The tissue inhibitors of metalloproteinases (TIMPs) are naturally occurring proteins that specifically inhibit matrix metalloproteinases and regulate extracellular matrix turnover and tissue remodeling by forming tightbinding inhibitory complexes with the MMPs. Thus, TIMPs maintain the balance between matrix destruction and formation. An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors. The TIMP proteins share several structural features including six loops held in place by six disulfide bonds arranged in three knot-like structures. These proteins also contain twelve cysteine residues in conserved regions of the molecule that form six disulfide bonds, essential for the formation of native conformations, and the N terminal region that is necessary for inhibitory activities. The N terminus of each TIMP contains a consensus sequence (VIRAK) and each TIMP is translated with a 29 amino acid leader sequence that is cleaved off to produce the mature protein. The C terminal regions are divergent, which enhances the selectivity of inhibition and binding efficiency. Although the TIMP proteins share high homology, they may either be secreted extracellularly in soluble form (TIMP1, TIMP2 and TIMP4) or bind to extracellular matrix components (TIMP3). The MMPs and TIMPs can be divided into two groups with respect to gene expression: the majority exhibit inducible expression and a small number are produced constitutively or are expressed at very low levels and are not inducible. Among agents that induce MMP and TIMP production are the inflammatory cytokines TNF alpha and IL1 beta. A marked cell type specificity is a hallmark of both MMP and TIMP gene expression (i.e. a limited number of cell types can be induced to make these proteins). Tissue Inhibitor of Metalloproteinases 4 (TIMP4) was identified by molecular cloning. TIMP4 shows 37% amino acid identity with TIMP1 and 51% homology with TIMP2 and TIMP3. TIMP4 is secreted extracellularly, predominantly in heart and brain tissue. It may function in a tissue specific fashion in extracellular matrix (ECM) homeostasis. TIMP4 has a strong inhibitory effect on the invasion of human breast cancer cells across reconstituted basement membranes suggesting that TIMP4 may have an important role in inhibiting primary tumor growth and progression. The human TIMP4 gene has the chromosomal location of 3p25.