Testing Data
Rabbit anti-Human TIMP-1 (Tissue Inhibitor of Metalloproteinases) Polyclonal Antibody | anti-TIMP-1 antibody
Anti-TIMP-1 (Tissue Inhibitor of Metalloproteinases) Loop 2 Antibody
Reactivity
Human
Applications
Western Blot
Purity
Immunogen Affinity Purified
Synonyms
TIMP-1 (Tissue Inhibitor of Metalloproteinases); Polyclonal Antibody; Anti-TIMP-1 (Tissue Inhibitor of Metalloproteinases) Loop 2 Antibody; anti-TIMP-1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
This antibody recognizes reduced TIMP1, but does not bind as well to the non reduced protein. It also does not cross react with other TIMP family members (TIMP2, TIMP3, TIMP4).
Purity/Purification
Immunogen Affinity Purified
Form/Format
Liquid; PBS, pH 7.4 with 0.05% sodium azide.
Concentration
1ug/ul (varies by lot)
Sequence Length
207
Applicable Applications for anti-TIMP-1 antibody
Western Blot (WB)
Application Notes
Anti-TIMP-1 works well for Western Blotting applications, and when used against the reduced protein identifies a band at 29 Kd. RP1T1 does not bind as well to the non-reduced protein, and has limited use for immunoprecipitation, immunohistochemistry, and ELISA applications. TIMP-1 is produced in low (pg/ml) levels by most cell types. Treatment of cells with the phorbol ester TPA stimulates production of TIMP-1 in some cell types, but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples. RP2T1 does not recognize murine or Rat TIMP-1 as well as it does human TIMP-1; RP3T1 works better for this application.
Immunogen
Synthetic peptide based on loop #2 and part of loop #1 of human TIMP1.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
Related Product Information for anti-TIMP-1 antibody
Description: The antibody is a Rabbit antibody to the Tissue Inhibitor of Metalloproteinases, TIMP-1, made with a synthetic peptide based on loop #2 and part of Loop #1of the Human sequence. The antibody binds to TIMP-1, but does not cross react with the other TIMP family members (TIMP-2, TIMP-3, TIMP-4). The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of Sodium Azide as preservative and 50% glycerol as cryoprotectant.
Relevance: Tissue inhibitor of metalloproteinases 1 (TIMP1) and Tissue inhibitor of metalloproteinases 2 (TIMP 2) have similar properties, specifically in inhibiting enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP1 forms a complex of 1:1 stoichiometry with activated interstitial collagenases, activated stromelysin, active form of 72kDa Type IV collagenase (also known as MMP2 or gelatinase A), and latent and active forms 92kDa Type IV collagenase (also known as MMP0 or gelatinase B). TIMPs inhibit the proteolytic invasiveness of tumour cells and normal placental trophoblast cells. TIMP1 is produced in low (pg/ml) levels by most cell types. Treatment of cells with the phorbol ester TPA stimulates production of TIMP1 in some cell types, but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting.
Relevance: Tissue inhibitor of metalloproteinases 1 (TIMP1) and Tissue inhibitor of metalloproteinases 2 (TIMP 2) have similar properties, specifically in inhibiting enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP1 forms a complex of 1:1 stoichiometry with activated interstitial collagenases, activated stromelysin, active form of 72kDa Type IV collagenase (also known as MMP2 or gelatinase A), and latent and active forms 92kDa Type IV collagenase (also known as MMP0 or gelatinase B). TIMPs inhibit the proteolytic invasiveness of tumour cells and normal placental trophoblast cells. TIMP1 is produced in low (pg/ml) levels by most cell types. Treatment of cells with the phorbol ester TPA stimulates production of TIMP1 in some cell types, but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting.
NCBI and Uniprot Product Information
Research Articles on TIMP-1
Similar Products
Product Notes
The TIMP-1 (Catalog #AAA4157369) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TIMP-1 (Tissue Inhibitor of Metalloproteinases) Loop 2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TIMP-1 (Tissue Inhibitor of Metalloproteinases) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Anti-TIMP-1 works well for Western Blotting applications, and when used against the reduced protein identifies a band at 29 Kd. RP1T1 does not bind as well to the non-reduced protein, and has limited use for immunoprecipitation, immunohistochemistry, and ELISA applications. TIMP-1 is produced in low (pg/ml) levels by most cell types. Treatment of cells with the phorbol ester TPA stimulates production of TIMP-1 in some cell types, but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples. RP2T1 does not recognize murine or Rat TIMP-1 as well as it does human TIMP-1; RP3T1 works better for this application. Researchers should empirically determine the suitability of the TIMP-1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TIMP-1 (Tissue Inhibitor of Metalloproteinases), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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