LETM1 Domain Containing Protein 1 Recombinant Protein | LETMD1 recombinant protein

Recombinant LETM1 Domain Containing Protein 1 (LETMD1)

Cat. Num. AAA2029893

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: LETM1 Domain Containing Protein 1; Recombinant LETM1 Domain Containing Protein 1 (LETMD1); LETMD1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; PRQLLIRHF WTPKQQTDFL DIYHAFRKQS HPEIISYLEKVIPLISDAGL RWRLTDLCTK IQRGTHPAIH DILALRECFS NHPLGMNQLQALHVKALSRA MLLTSYLPPP LLRHRLKTHT TVIHQLDKAL AKLGIGQLTAQEVKSACYLR GLNSTHIGED RCRTWLGEWL QISCSLKEAE LSLLLHN
• Sequence Length: 739

• Applicable Applications for LETMD1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 25.1kDa
• Accurate Molecular Mass (KD): 25kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Pro162~Asn347
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6912482
• NCBI GeneID: 3954
• NCBI Accession #: NP_036450.1
• NCBI GenBank Nucleotide #: NM_012318.2
• Molecular Weight: 33,245 Da
• NCBI Official Full Name: LETM1 and EF-hand domain-containing protein 1, mitochondrial
• NCBI Official Synonym Full Names: leucine zipper-EF-hand containing transmembrane protein 1
• NCBI Official Symbol: LETM1
• NCBI Protein Information: LETM1 and EF-hand domain-containing protein 1, mitochondrial
• UniProt Protein Name: LETM1 and EF-hand domain-containing protein 1, mitochondrial
• Protein Family: LETM1 domain-containing protein
• UniProt Gene Name: LETM1
• UniProt Entry Name: LETM1_HUMAN

NCBI Description

This gene encodes a protein that is localized to the inner mitochondrial membrane. The protein functions to maintain the mitochondrial tubular shapes and is required for normal mitochondrial morphology and cellular viability. Mutations in this gene cause Wolf-Hirschhorn syndrome, a complex malformation syndrome caused by the deletion of parts of the distal short arm of chromosome 4. Related pseudogenes have been identified on chromosomes 8, 15 and 19. [provided by RefSeq, Oct 2009]

Uniprot Description

LETM1: Crucial for the maintenance of mitochondrial tubular networks and for the assembly of the supercomplexes of the respiratory chain. Required for the maintenance of the tubular shape and cristae organization. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Calcium-binding; Mitochondrial; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: mitochondrion; mitochondrial inner membrane; integral to membrane

Molecular Function: protein binding; calcium ion binding

Biological Process: cristae formation

Disease: Wolf-hirschhorn Syndrome