Immunohistochemistry (IHC)
(TIMP4 (5ug/ml) was used in IHC to stain ormalin-fixed, paraffin-embedded human uterus, endometrium, followed by biotinylated goat,r abbit IgG, alkaline phosphatase-streptavidin and chromogen.)
Rabbit TIMP-4 Polyclonal Antibody | anti-TIMP4 antibody
TIMP-4, CT (TIMP4, Tissue Inhibitor of Metalloproteinases 4)
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
TIMP-4; Polyclonal Antibody; CT (TIMP4; Tissue Inhibitor of Metalloproteinases 4); Anti -TIMP-4; anti-TIMP4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human TIMP4. Species Crossreactivity: Mouse, rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Applicable Applications for anti-TIMP4 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: ELISA: 1:20,000
Western Blot: 1:500-1:1000
Immunohistochemistry (Paraffin): 5ug/ml
Dilution: ELISA: 1:20,000
Western Blot: 1:500-1:1000
Immunohistochemistry (Paraffin): 5ug/ml
Immunogen
Synthetic peptide corresponding to human TIMP4 at C-Terminal.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunohistochemistry (IHC)
(TIMP4 (5ug/ml) was used in IHC to stain ormalin-fixed, paraffin-embedded human uterus, endometrium, followed by biotinylated goat,r abbit IgG, alkaline phosphatase-streptavidin and chromogen.)
Immunohistochemistry (IHC)
(TIMP4 (5ug/ml) was used in IHC to stain ormalin-fixed, paraffin-embedded human uterus, endometrium, followed by biotinylated goat,r abbit IgG, alkaline phosphatase-streptavidin and chromogen.)
Western Blot (WB)
(Western blot analysis of extracts from NIH-3T3 cells, using TIMP4. The lane on the right is treated with the synthesized peptide)
Western Blot (WB)
(Western blot analysis of extracts from NIH-3T3 cells, using TIMP4. The lane on the right is treated with the synthesized peptide)
Related Product Information for anti-TIMP4 antibody
The matrix metalloproteinases (MMPs) are a large family of zinc endopeptidases that degrade extracellular matrix components such as fibronectin and collagen type III. The tissue inhibitors of metalloproteinase (TIMPs) complex with these matrix metalloproteinases and irreversibly inactivate them. The TIMP proteins are homologous and are either secreted in soluble form (TIMP1, TIMP2 and TIMP4) or bind to extracellular matrix components (TIMP3). Metalloproteinase inhibitor 4 (TIMP4) is known to inhibit MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9.
Product Categories/Family for anti-TIMP4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
25,593 Da
NCBI Official Full Name
TPA: metalloproteinase inhibitor 4
NCBI Official Synonym Full Names
TIMP metallopeptidase inhibitor 4
NCBI Official Symbol
TIMP4
NCBI Protein Information
metalloproteinase inhibitor 4; TIMP-4; tissue inhibitor of metalloproteinase; tissue inhibitor of metalloproteinases 4
UniProt Protein Name
Metalloproteinase inhibitor 4
Protein Family
UniProt Gene Name
TIMP4
UniProt Synonym Gene Names
TIMP-4
UniProt Entry Name
TIMP4_BOVIN
Uniprot Description
Function: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor
By similarity.
Subcellular location: Secreted.
Sequence similarities: Belongs to the protease inhibitor I35 (TIMP) family. [View classification]Contains 1 NTR domain.
Similar Products
Product Notes
The TIMP4 timp4 (Catalog #AAA643795) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TIMP-4, CT (TIMP4, Tissue Inhibitor of Metalloproteinases 4) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TIMP-4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot and Immunohistochemistry. Dilution: ELISA: 1:20,000 Western Blot: 1:500-1:1000 Immunohistochemistry (Paraffin): 5ug/ml. Researchers should empirically determine the suitability of the TIMP4 timp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TIMP-4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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