Rabbit anti-Human TIMP-3 (Tissue Inhibitor of Metalloproteinases) Polyclonal Antibody | anti-TIMP-3 antibody

Anti-TIMP-3 (Tissue Inhibitor of Metalloproteinases) Loop 3 Antibody

Cat. Num. AAA4157378

• Gene Names: TIMP3; SFD; K222; K222TA2; HSMRK222
• Reactivity: Human
• Applications: Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: TIMP-3 (Tissue Inhibitor of Metalloproteinases); Polyclonal Antibody; Anti-TIMP-3 (Tissue Inhibitor of Metalloproteinases) Loop 3 Antibody; anti-TIMP-3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: The antibody binds to TIMP3, but does not cross react with the other TIMP family members (TIMP1, TIMP2, TIMP4). The antibody recognizes the glycosylated and unglycosylated forms of TIMP3, and reacts with native or reduced TIMP3.
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 211

• Applicable Applications for anti-TIMP-3 antibody: Western Blot (WB)
• Application Notes: Anti-TIMP-3 works well for Western Blotting applications, and when used against the reduced protein identifies bands at approximately 24 Kd and 30 Kd (unglycosylated and glycosylated, respectively). RP3T3 recognizes both reduced and nonreduced TIMP-3. TIMP-3 is produced in low (pg/ml) levels by most cell types, and appears to be preferentially secreted into the extracellular matrix. For Western blots and reverse zymogram analysis, an ECM preparation is most often used, but a total cell lysate can also be used. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates.
• Immunogen: Synthetic peptide based on Loop 3 of Human TIMP3.
• Cellular Localization: Secreted; Extracellular Space; Extracellular Matrix.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-TIMP-3 antibody

Description: The antibody is a Rabbit antibody to the Tissue Inhibitor of Metalloproteinases, TIMP-3, made with a synthetic peptide based on Loop #3 of the Human sequence. The antibody binds to TIMP-3, but does not cross react with the other TIMP family members (TIMP-1, TIMP-2, TIMP-4). RP3T3 recognizes the glycosylated and unglycosylated forms of TIMP-3, and works against native or reduced TIMP-3. The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, 50% glycerol, with the addition of Sodium Azide as preservative.

Relevance: The tissue inhibitors of metalloproteinases (TIMPs) are naturally occurring proteins that specifically inhibit matrix metalloproteinases and regulate extracellular matrix turnover and tissue remodeling by forming tightly bound inhibitory complexes with the MMPs. Thus, TIMPs maintain the balance between matrix destruction and formation. An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors. TIMP proteins share several structural features including six loops held in place by six disulfide bonds arranged in three knotlike structures. The N terminal region is necessary for inhibitory activity. The N terminus of each TIMP contains a consensus sequence (VIRAK) and each TIMP is translated with a 29 amino acid leader sequence that is cleaved to produce the mature protein. The C terminal regions are divergent and enhance the inhibition selectivity and binding efficiency. Although the TIMP proteins share high homology, following secretion they are localized extracellularly either in soluble form (TIMP1, TIMP2, and TIMP4) or bound to extracellular matrix components (TIMP3). The MMPs and TIMPs can be divided into two groups with respect to gene expression: the majority exhibit inducible expression and a small number are produced constitutively or are expressed at very low levels and are not inducible. Among agents that induce MMP and TIMP production are the inflammatory cytokines TNF alpha and IL beta. A marked cell type specificity is a hallmark of both MMP and TIMP gene expression (i.e. a limited number of cell types can be induced to make these proteins). Tissue Inhibitor of Metalloproteinase 3 (TIMP3) was first purified from chicken embryo fibroblasts and identified as ChIMP3. The human homologue of TIMP3, was originally detected as an inducible serum protein in WI-38 fibroblasts. The TIMP3 localization differs from that of the other three TIMPs, and is thought to be primarily deposited into the extracellular matrix (ECM). TIMP3 is insoluble, binds to the ECM associated with a variety of cell types, and is widely distributed throughout the body. TIMP3 shows 30% amino acid homology with TIMP1 and 38% homology with TIMP2. TIMP3 has been shown to promote the detachment of transformed cells from ECM and to accelerate morphological changes associated with cell transformation. Furthermore, up regulation of TIMP 3 has been associated with a block in the G1 phase of the cell cycle during differentiation of HL60 leukemia cells. The human TIMP3 gene has the chromosomal location of 22q12-22q13. TIMP3 mRNA is highly expressed in placenta but is also found in the heart, kidney, lung, pancreas, uterus and skeletal muscle with low levels in the brain. In endometrium, TIMP3 is reported to be expressed in luminal epithelium, glands, stroma, endothelial cells and vascular smooth muscle cells. TIMP3 is also reported to be expressed by fibroblast-like cells in ulcerated intestinal wall

NCBI and Uniprot Product Information

• NCBI GI #: 998826
• NCBI GeneID: 7078
• NCBI Official Full Name: TIMP-3
• NCBI Official Synonym Full Names: TIMP metallopeptidase inhibitor 3
• NCBI Official Symbol: TIMP3
• NCBI Official Synonym Symbols: SFD; K222; K222TA2; HSMRK222
• NCBI Protein Information: metalloproteinase inhibitor 3

NCBI Description

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy. [provided by RefSeq, Jul 2008]