anti-Human, Rat TCPTP Polyclonal Antibody | anti-TCPTP antibody

Anti-TCPTP Antibody

Cat. Num. AAA177778

• Gene Names: PTPN2; PTN2; PTPT; TCPTP; TC-PTP; TCELLPTP
• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: TCPTP; Polyclonal Antibody; Anti-TCPTP Antibody; Tyrosine-protein phosphatase non-receptor type 2; Protein tyrosine phosphatase non receptor type 2; PTN2; PTN2_HUMAN; PTPN 2; PTPN2; PTPT; T cell protein tyrosine phosphatase; T-cell protein-tyrosine phosphatase; TC PTP; TCELLPTP; Tyrosine protein phosphatase non receptor type 2; protein tyrosine phosphatase; non-receptor type 2; anti-TCPTP antibody

• Reactivity: Human, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 410

• Applicable Applications for anti-TCPTP antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: E Coli-derived human TCPTP recombinant protein (Position: M1-C330). Human TCPTP shares 91.4% and 92% amino acid (aa) sequence identity with mouse and rat TCPTP, respectively.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- TCPTP Picoband antibody, MBS177778, Western blottingAll lanes: Anti TCPTP (MBS177778) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: JURKAT Whole Cell Lysate at 40ugPredicted bind size: 48KDObserved bind size: 48KD )

Immunohistochemistry (IHC)

(Anti- TCPTP Picoband antibody, MBS177778,IHC(P)IHC(P): Rat Kidney Tissue )

Immunohistochemistry (IHC)

(Anti- TCPTP Picoband antibody, MBS177778,IHC(P)IHC(P): Human Intestinal Cancer Tissue )

Related Product Information for anti-TCPTP antibody

Description: Rabbit IgG polyclonal antibody for Tyrosine-protein phosphatase non-receptor type 2(PTPN2) detection. Tested with WB, IHC-P in Human;Rat.

Background: TCPTP is also called as PTPN2. The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. Multiple alternatively spliced transcript variants encoding different isoforms have been found. Two highly related but distinctly processed pseudogenes that localize to chromosomes 1 and 13, respectively, have been reported.

References

1. "Entrez Gene: PTPN2 protein tyrosine phosphatase, non-receptor type 2". 2. Brown-Shimer S, Johnson KA, Lawrence JB, Johnson C, Bruskin A, Green NR, Hill DE (Aug 1990). "Molecular cloning and chromosome mapping of the human gene encoding protein phosphotyrosyl phosphatase 1B". Proc Natl Acad Sci U S A 87 (13): 5148-52.

NCBI and Uniprot Product Information

• NCBI GI #: 333108235
• NCBI GeneID: 5771
• NCBI Accession #: NP_001193942.1
• NCBI GenBank Nucleotide #: NM_001207013.1
• UniProt Accession #: P17706; Q96AU5; Q96HR2; A8K955; A8MXU3; K7ENG3
• Molecular Weight: 48,017 Da
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 2 isoform 4
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 2
• NCBI Official Symbol: PTPN2
• NCBI Official Synonym Symbols: PTN2; PTPT; TCPTP; TC-PTP; TCELLPTP
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 2
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 2
• UniProt Gene Name: PTPN2
• UniProt Synonym Gene Names: PTPT; TCPTP
• UniProt Entry Name: PTN2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. Multiple alternatively spliced transcript variants encoding different isoforms have been found. Two highly related but distinctly processed pseudogenes that localize to chromosomes 1 and 13, respectively, have been reported. [provided by RefSeq, May 2011]

Uniprot Description

PTPN2: a type 2 non-receptor phospho-tyrosine-protein phosphatase (PTP). Two alternatively spliced isoforms are differenyially expressed. Isoform A is a major PTP expressed in human tissues, while isoform B is expressed mainly in T-cells and in placenta.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, tyrosine (non-receptor); EC 3.1.3.48

Chromosomal Location of Human Ortholog: 18p11.3-p11.2

Cellular Component: endoplasmic reticulum; ER-Golgi intermediate compartment; nucleoplasm; nucleus; plasma membrane

Molecular Function: integrin binding; non-membrane spanning protein tyrosine phosphatase activity; protein binding; protein kinase binding; protein tyrosine phosphatase activity; receptor tyrosine kinase binding; syntaxin binding

Biological Process: B cell differentiation; erythrocyte differentiation; glucose homeostasis; insulin receptor signaling pathway; negative regulation of cell proliferation; negative regulation of chemotaxis; negative regulation of epidermal growth factor receptor signaling pathway; negative regulation of inflammatory response; negative regulation of insulin receptor signaling pathway; negative regulation of macrophage differentiation; negative regulation of T cell receptor signaling pathway; negative regulation of tyrosine phosphorylation of Stat1 protein; negative regulation of tyrosine phosphorylation of Stat3 protein; negative regulation of tyrosine phosphorylation of Stat5 protein; negative regulation of tyrosine phosphorylation of Stat6 protein; positive regulation of gluconeogenesis; T cell differentiation