Western Blot (WB)
(Western blot analysis of RHOT1 in rat brain tissue lysate with RHOT1 antibody at 1 μg/ml.)
Rabbit RHOT1 Polyclonal Antibody | anti-RHOT1 antibody
RHOT1 Antibody
Gene Names
RHOT1; ARHT1; MIRO1; MIRO-1
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
RHOT1 antibody is affinity chromatography purified via peptide column.
Synonyms
RHOT1; Polyclonal Antibody; RHOT1 Antibody; Ras homolog family member T1; mitochondrial Rho GTPase 1; ARHT1; MIRO-1; MIRO1; MIRO; ras homolog family member T1; anti-RHOT1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
RHOT1 antibody is human, mouse and rat reactive. At least three isoforms of RHOT1 are known to exist; this antibody will detect all three isoforms.
Purity/Purification
RHOT1 antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid; RHOT1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1 mg/mL (varies by lot)
Sequence Length
691
Applicable Applications for anti-RHOT1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
RHOT1 antibody can be used for detection of RHOT1 by Western blot at 1 - 2 ug/ml. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL.
Conjugate
Unconjugated
Immunogen
RHOT1 antibody was raised against a 15 amino acid peptide near the amino terminus of human RHOT1.
The immunogen is located within amino acids 40 - 90 of RHOT1.
The immunogen is located within amino acids 40 - 90 of RHOT1.
Homology
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken:(81%)
Note
Optimal dilutions for each applicationto be determined by the researcher.
Preparation and Storage
RHOT1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
Western Blot (WB)
(Western blot analysis of RHOT1 in rat brain tissue lysate with RHOT1 antibody at 1 μg/ml.)
Western Blot (WB)
(Western blot analysis of RHOT1 in rat brain tissue lysate with RHOT1 antibody at 1 μg/ml.)
Immunohistochemistry (IHC)
(Immunohistochemistry of RHOT in mouse brain tissue with RHOT antibody at 5 μg/ml.)
Immunohistochemistry (IHC)
(Immunohistochemistry of RHOT in mouse brain tissue with RHOT antibody at 5 μg/ml.)
Immunofluorescence (IF)
(Immunofluorescence of RHOT in mouse brain tissue with RHOT antibody at 20 μg/ml.)
Immunofluorescence (IF)
(Immunofluorescence of RHOT in mouse brain tissue with RHOT antibody at 20 μg/ml.)
Related Product Information for anti-RHOT1 antibody
The Ras homolog family member T1 (RHOT) is an atypical Rho Ca2+-binding GTPase that localizes to the mitochondria (1). RHOT1, the related protein RHOT2, the adaptor protein Milton, and the PTEN induced putative kinase 1 (PINK1), form a complex that is involved in axonal transport of mitochondria (2,3). Both PINK1 and Parkin target RHOT1 for phosphorylation and degradation, causing the arrest of mitochondrial motility (4).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted: 76 kDa
Observed: 78 kDa
Observed: 78 kDa
NCBI Official Full Name
mitochondrial Rho GTPase 1 isoform 1
NCBI Official Synonym Full Names
ras homolog family member T1
NCBI Official Symbol
RHOT1
NCBI Official Synonym Symbols
ARHT1; MIRO1; MIRO-1
NCBI Protein Information
mitochondrial Rho GTPase 1; mitochondrial Rho (MIRO) GTPase 1; ras homolog gene family, member T1; rac-GTP binding protein-like protein
UniProt Protein Name
Mitochondrial Rho GTPase 1
UniProt Gene Name
RHOT1
UniProt Synonym Gene Names
ARHT1; MIRO-1; hMiro-1
UniProt Entry Name
MIRO1_HUMAN
Uniprot Description
RHOT1: Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution. Belongs to the mitochondrial Rho GTPase family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: G protein, monomeric; Membrane protein, integral; Hydrolase; Mitochondrial; G protein, monomeric, Rho; EC 3.6.5.-; G protein
Chromosomal Location of Human Ortholog: 17q11.2
Cellular Component: membrane; plasma membrane; cytosol; integral to mitochondrial outer membrane
Molecular Function: GTPase activity; protein binding; GTP binding; calcium ion binding
Biological Process: regulation of small GTPase mediated signal transduction; metabolic process; small GTPase mediated signal transduction; cellular homeostasis; mitochondrion transport along microtubule
Research Articles on RHOT1
Similar Products
Product Notes
The RHOT1 rhot1 (Catalog #AAA153364) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RHOT1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RHOT1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). RHOT1 antibody can be used for detection of RHOT1 by Western blot at 1 - 2 ug/ml. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Researchers should empirically determine the suitability of the RHOT1 rhot1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RHOT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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