Rabbit anti-Human mTOR Polyclonal Antibody | anti-mTOR antibody

mTOR (Phospho-Ser2448) Antibody

Cat. Num. AAA9406487

• Gene Names: MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: mTOR; Polyclonal Antibody; mTOR (Phospho-Ser2448) Antibody; FRAP; FRAP1; FRAP2; RAFT1; anti-mTOR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of mTOR only when phosphorylated at serine 2448.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 2549

• Applicable Applications for anti-mTOR antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500~1:1000; Immunohistochemistry: 1:50~1:100; Immunofluorescence: 1:100~1:200
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of serine 2448 (T-D-S(p)-Y-S) derived from Human mTOR.
• Modification: Phospho-Ser2448
• Target Name: mTOR
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Western Blot (WB)

(Western blot analysis of extracts from Hela cells, treatedwith IFNa or calf intestinal phosphatase (CIP), using mTOR(Phospho-Ser2448) Antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using mTOR(Phospho-Ser2448) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed MCF cells using mTOR(Phospho-Ser2448) Antibody.)

Western Blot (WB)

(Western blot analysis of extracts from 293 cells untreatedor treated with PMA using mTOR(Phospho-Ser2448) Antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis ofparaffin-embedded human breast carcinomatissue using mTOR (Phospho-Ser2448) Antibody)

Related Product Information for anti-mTOR antibody

Kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.

Product Categories/Family for anti-mTOR antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4826730
• NCBI GeneID: 2475
• NCBI Accession #: NP_004949.1
• NCBI GenBank Nucleotide #: NM_004958.3
• UniProt Accession #: P42345; Q4LE76; Q5TER1; Q6LE87; Q96QG3; Q9Y4I3
• Molecular Weight: 288,892 Da
• NCBI Official Full Name: serine/threonine-protein kinase mTOR
• NCBI Official Synonym Full Names: mechanistic target of rapamycin (serine/threonine kinase)
• NCBI Official Symbol: MTOR
• NCBI Official Synonym Symbols: FRAP; FRAP1; FRAP2; RAFT1; RAPT1
• NCBI Protein Information: serine/threonine-protein kinase mTOR
• UniProt Protein Name: Serine/threonine-protein kinase mTOR
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: MTOR
• UniProt Synonym Gene Names: FRAP; FRAP1; FRAP2; RAFT1; RAPT1; mTOR
• UniProt Entry Name: MTOR_HUMAN

NCBI Description

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

mTOR: an atypical kinase belonging to the PIKK family of kinases. Is the catalytic subunit of two protein complexes, mTORC1 and mTORC2. mTORC1 activates S6K and inactivates 4E-BP1, up-regulating protein synthesis. mTORC1 contains Raptor, a positive regulatory subunit and scaffold for recruiting substrates, two negative regulators, PRAS40 and DEPTOR, and mLST8; it is a target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. mTORC2, a downstream effector of PI3K, is insensitive to rapamycin and activates Akt by phosphorylating a key activation site. mTORC2 contains regulatory subunits Rictor and mSIN1, PROTOR, mLST8, and the negative regulator DEPTOR. mTORC1 suppresses PI3K activity via a strong negative feedback loop that involves S6K1. Inhibiting mTORC1 ablates this negative feedback loop and potentiates PI3K signaling. Known inhibitors of mTOR include rapamycin, temsirolimus (CCI-779).

Protein type: EC 2.7.11.1; Autophagy; Motility/polarity/chemotaxis; Protein kinase, atypical; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); ATYPICAL group; PIKK family; FRAP subfamily

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: PML body; endoplasmic reticulum membrane; lysosomal membrane; lysosome; dendrite; endomembrane system; cytosol; nucleoplasm; Golgi membrane; mitochondrial outer membrane; cell soma; membrane; cytoplasm; TORC2 complex; nucleus; phosphoinositide 3-kinase complex

Molecular Function: protein dimerization activity; protein serine/threonine kinase activity; protein domain specific binding; protein binding; phosphoprotein binding; ribosome binding; drug binding; kinase activity; ATP binding

Biological Process: negative regulation of autophagy; regulation of myelination; TOR signaling pathway; positive regulation of translation; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; regulation of glycogen biosynthetic process; negative regulation of cell size; signal transduction; protein amino acid phosphorylation; germ cell development; negative regulation of macroautophagy; cellular response to nutrient levels; positive regulation of stress fiber formation; regulation of carbohydrate utilization; response to stress; protein catabolic process; cell growth; response to nutrient; regulation of response to food; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; peptidyl-threonine phosphorylation; positive regulation of lipid biosynthetic process; response to amino acid stimulus; negative regulation of NFAT protein import into nucleus; double-strand break repair via homologous recombination; positive regulation of protein kinase B signaling cascade; peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of actin filament polymerization; regulation of actin cytoskeleton organization and biogenesis; T cell costimulation; insulin receptor signaling pathway; ruffle organization and biogenesis; regulation of fatty acid beta-oxidation; positive regulation of transcription from RNA polymerase III promoter; innate immune response; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; regulation of protein kinase activity; vascular endothelial growth factor receptor signaling pathway; phosphorylation; growth