Rabbit Rubicon Polyclonal Antibody | anti-KIAA0226 antibody

Rubicon Antibody

Cat. Num. AAA153396

• Gene Names: KIAA0226; SCAR15; RUBICON
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Rubicon antibody is affinity chromatography purified via peptide column.
• Synonyms: Rubicon; Polyclonal Antibody; Rubicon Antibody; Run domain Beclin-1 interacting and cysteine-rich containing protein; KIAA0226; anti-KIAA0226 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Rubicon antibody is human, mouse and rat reactive. Multiple isoforms of Rubicon are known to exist.
• Purity/Purification: Rubicon antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 987

• Applicable Applications for anti-KIAA0226 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-p)
• Application Notes: Optimal dilutions for each application to be determined by the researcher.
• Conjugate: Unconjugated
• Immunogen: Rubicon antibody was raised against a 16 amino acid peptide near the amino terminus of human Rubicon.; The immunogen is located within amino acids 240-290 of Rubicon.
• Buffer: Rubicon antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: Rubicon antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of Rubicon in 293 cell lysate with Rubicon antibody at 1 μg/ml.)

Immunohistochemistry (IHC)

(Immunohistochemistry of RUBICON in human kidney tissue with RUBICON antibody at 5 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of RUBICON in human kidney tissue with RUBICON antibody at 20 μg/ml.)

Related Product Information for anti-KIAA0226 antibody

Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon caused enhancement of autophagy while that of ATG14L caused a defect in autophagosome formation (1). Rubicon functions as part of a Beclin-1-PIK3C3-containing autophagy complex and is also an essential, positive regulator of the NAPDH oxidase complex (2). Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the NAPDH oxidase complex, leading to a burst of reactive oxygen species and inflammatory cytokines (2).

NCBI and Uniprot Product Information

• NCBI GI #: 530375960
• NCBI GeneID: 9711
• NCBI Accession #: XP_005269431
• NCBI GenBank Nucleotide #: XM_005269374.1
• Molecular Weight: Predicted: 90; 101; 109 kDa; ; Observed: 91; 99 kDa
• NCBI Official Full Name: run domain Beclin-1 interacting and cysteine-rich containing protein isoform X1
• NCBI Official Synonym Full Names: KIAA0226
• NCBI Official Symbol: KIAA0226
• NCBI Official Synonym Symbols: SCAR15; RUBICON
• NCBI Protein Information: run domain Beclin-1 interacting and cysteine-rich containing protein; baron; rundataxin; beclin-1 associated RUN domain containing protein; run domain Beclin-1 interacting and cystein-rich containing protein; RUN domain protein as Beclin 1-interacting and cysteine-rich containing; RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein
• UniProt Protein Name: Run domain Beclin-1 interacting and cysteine-rich containing protein
• UniProt Gene Name: KIAA0226
• UniProt Synonym Gene Names: Rubicon; Baron
• UniProt Entry Name: RUBIC_HUMAN

NCBI Description

The protein encoded by this gene is a negative regulator of autophagy and endocytic trafficking and controls endosome maturation. This protein contains two conserved domains, an N-terminal RUN domain and a C-terminal DUF4206 domain. The RUN domain is involved in Ras-like GTPase signaling, and the DUF4206 domain contains a diacylglycerol (DAG) binding-like motif. Mutation in this gene results in deletion of the DAG binding-like motif and causes a recessive ataxia. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Apr 2014]

Uniprot Description

rubicon: Negatively regulates endocytic trafficking. Impairs the autophagosome maturation process. May inhibit PIK3C3 activity. Forms a complex with BECN1, PIK3C3, PIK3R4 and UVRAG, but not with ATG14. The complex containing ATG14 up-regulates autophagy, while the one containing Rubicon down-regulates autophagy. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: Golgi apparatus; lysosome; late endosome; early endosome

Molecular Function: protein binding

Biological Process: negative regulation of autophagy; negative regulation of endocytosis; autophagy; endocytosis

Disease: Spinocerebellar Ataxia, Autosomal Recessive 15