Rabbit KLF17 Polyclonal Antibody | anti-KLF17 antibody

KLF17 Antibody

Cat. Num. AAA153351

• Gene Names: KLF17; ZNF393; Zfp393
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: KLF17 antibody is affinity chromatography purified via peptide column.
• Synonyms: KLF17; Polyclonal Antibody; KLF17 Antibody; Kruppel-like factor 17; zinc finger protein 393; ZNF393; Zfp393; anti-KLF17 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: KLF17 antibody is human, mouse and rat reactive. At least two isoforms of KLF17 are known to exist; this antibody will only detect the large isoform. KLF17 antibody is predicted to not cross-react with other KLF proteins.
• Purity/Purification: KLF17 antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 389

• Applicable Applications for anti-KLF17 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: KLF17 antibody can be used for detection of KLF17 by Western blot at 1 - 2 mug/ml. Antibody can also be used for immunohistochemistry starting at 5 mug/mL.
• Conjugate: Unconjugated
• Immunogen: KLF17 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human KLF17.
• Buffer: KLF17 antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: KLF17 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of KLF17 in rat liver tissue lysate with KLF17 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)

Immunohistochemistry (IHC)

(Immunohistochemistry of KLF17 in mouse liver tissue with KLG17 antibody at 5 μg/ml.)

Related Product Information for anti-KLF17 antibody

KLF17, also known as ZNF393, is a member of the Sp/KLF family of transcription factors (1,2). KLF17 binds to the promoter and negatively regulates the transcription of Id-1, a key metastasis regulator in breast cancer (3). Suppression of KLF17 promotes breast cancer cell invasion and epithelial-mesenchymal transition (3). Down-regulated KLF17 expression is also associated tumor invasion and poor prognosis in hepatocellular carcinoma, suggesting that the level of KLF17 expression can serve as a prognostic indicator (4).

NCBI and Uniprot Product Information

• NCBI GI #: 104294874
• NCBI GeneID: 128209
• NCBI Accession #: NP_775755
• NCBI GenBank Nucleotide #: NM_173484.3
• UniProt Accession #: Q5JT82; Q86VQ7; Q8N805
• Molecular Weight: Predicted: 28; 39; 43 kDa; ; Observed: 38 kDa
• NCBI Official Full Name: Krueppel-like factor 17
• NCBI Official Synonym Full Names: Kruppel-like factor 17
• NCBI Official Symbol: KLF17
• NCBI Official Synonym Symbols: ZNF393; Zfp393
• NCBI Protein Information: Krueppel-like factor 17; zinc finger protein 393; novel zinc-finger protein
• UniProt Protein Name: Krueppel-like factor 17
• Protein Family: Krueppel-like factor
• UniProt Gene Name: KLF17
• UniProt Synonym Gene Names: ZNF393
• UniProt Entry Name: KLF17_HUMAN

Uniprot Description

KLF17: Transcription repressor that binds to the promoter of target genes and prevents their expression. Acts as a negative regulator of epithelial-mesenchymal transition and metastasis in breast cancer. Specifically binds the 5'-CACCC-3' sequence in the promoter of ID1, a key metastasis regulator in breast cancer, and repress its expression. May be a germ cell-specific transcription factor that plays important roles in spermatid differentiation and oocyte development. Belongs to the Sp1 C2H2-type zinc-finger protein family.

Protein type: DNA-binding; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: nucleus

Molecular Function: metal ion binding; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; gamete generation