Rabbit INPP4B Polyclonal Antibody | anti-INPP4B antibody

INPP4B Antibody

Cat. Num. AAA154378

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Affinity chromatography purified via peptide column.
• Synonyms: INPP4B; Polyclonal Antibody; INPP4B Antibody; Inositol polyphosphate-4-phosphatase type II 105kDa; anti-INPP4B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: INPP4B antibody is human, mouse and rat reactive. At least two isoforms of INPP4B are known to exist; this antibody only detects the longer isoform. INPP4B antibody is predicted to not cross-react with INPP4A.
• Purity/Purification: Affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 924

• Applicable Applications for anti-INPP4B antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: INPP4B antibody can be used for detection of INPP4B by Western blot at 1 - 2 mug/ml. Antibody can also be used for immunocytochemistry starting at 2.5 mug/mL.
• Immunogen: INPP4B antibody was raised against a 17 amino acid peptide near the amino terminus of human INPP4B.
• Buffer: Supplied in PBS containing 0.02% sodium azide.
• Positive Control 1: 3T3 Cell Lysate
• Conjugate: Unconjugated
• Preparation and Storage: Store at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of INPP4B in 3T3 cell lysate with INPP4B antibody at 1 μg/ml.)

Immunocytochemistry (IHC)

(Immunocytochemistry of INPP4B in 3T3 cells with INPP4B antibody at 2.5 μg/ml.)

Related Product Information for anti-INPP4B antibody

Inositol polyphosphate 4-phosphatase type II (INPP4B) is one of the enzymes involved in phosphatidylinositol signaling pathways. It removes the phosphate group at position 4 of the inositol ring from inositol 3,4-bisphosphate (1). INPP4B can function as a tumor suppressor by inhibiting PI3K signaling. In human epithelial cells, knockdown of INPP4B expression resulted in enhanced Akt activation, anchorage-independent growth, and enhanced overall motility (2). It is thought INPP4B may also have oncogenic functions as INPP4B-dependent PI3K signaling is mediated by SGK3 in breast cancer cells and expression of INPP4B leads to enhanced SGK3 activation and suppression of AKT phosphorylation (3).

Product Categories/Family for anti-INPP4B antibody

Cancer; Cell Cycle

References

Norris FA, Auethavekiat V, and Majerus PW. The isolation and characterization of cDNA encoding human and rat brain inositol polyphosphate 4-phosphase. J. Biol. Chem. 1995; 270:16128-33. Gewinner C, Wang ZC, Richardson A, et al. Evidence that inositol polyphosphate 4-phosphase type II is a tumor suppressor that inhibits PI3K signaling. Cancer Cell 2009; 16:115-25. Gasser JA, Inuzuka H, Lau AW, et al. SGK3 mediates INPP4B-dependent PI3K signaling in breast cancer. Mol. Cell 2014; 56:595-607.

NCBI and Uniprot Product Information

• NCBI GI #: 156104895
• NCBI GeneID: 8821
• NCBI Accession #: NP_003857
• NCBI GenBank Nucleotide #: NM_003866.2
• UniProt Accession #: Q9BS68; Q2TAI2; Q5XLE7; Q6IN59; Q6PJB4; O15327
• Molecular Weight: Predicted: 102 kDa; Observed: 100 kDa
• NCBI Official Full Name: type II inositol 3,4-bisphosphate 4-phosphatase
• NCBI Official Synonym Full Names: inositol polyphosphate-4-phosphatase type II B
• NCBI Official Symbol: INPP4B
• NCBI Protein Information: type II inositol 3,4-bisphosphate 4-phosphatase
• UniProt Protein Name: Type II inositol 3,4-bisphosphate 4-phosphatase
• Protein Family: Type II inositol 3,4-bisphosphate 4-phosphatase
• UniProt Gene Name: INPP4B
• UniProt Entry Name: INP4B_HUMAN

NCBI Description

INPP4B encodes the inositol polyphosphate 4-phosphatase type II, one of the enzymes involved in phosphatidylinositol signaling pathways. This enzyme removes the phosphate group at position 4 of the inositol ring from inositol 3,4-bisphosphate. There is limited data to suggest that the human type II enzyme is subject to alternative splicing, as has been established for the type I enzyme. [provided by RefSeq, Jul 2008]

Uniprot Description

INPP4B: Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4- trisphosphate and inositol 1,4-bisphosphate. Widely expressed with highest levels occurring in the skeletal muscle and heart. Strongly inhibited by inositol hexakisphosphate. Belongs to the inositol 3,4-bisphosphate 4-phosphatase family.

Protein type: Carbohydrate Metabolism - inositol phosphate; Phosphatase, lipid; EC 3.1.3.66; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q31.21

Cellular Component: cytoplasm; cytosol

Molecular Function: inositol-1,3,4-trisphosphate 4-phosphatase activity; phosphatidylinositol-3,4-bisphosphate 4-phosphatase activity; protein binding

Biological Process: dephosphorylation; inositol phosphate metabolic process; phosphatidylinositol biosynthetic process; signal transduction