Western Blot (WB)
(Western blot analysis of IL-22 in HeLa cell lysate with IL-22 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide. )
Rabbit IL-22 Polyclonal Antibody | anti-IL22 antibody
IL-22 Antibody
Gene Names
IL22; TIFa; IL-21; IL-22; ILTIF; IL-TIF; IL-D110; zcyto18; TIFIL-23
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
IL-22 antibody is affinity chromatography purified via peptide column.
Synonyms
IL-22; Polyclonal Antibody; IL-22 Antibody; TIFa; IL-21; ILTIF; IL-TIF; IL-D110; zcyto18; TIFIL-23; ZCYTO18; UNQ3099/PRO10096; Interleukin-22; Cytokine Zcyto18; interleukin 22; anti-IL22 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
IL-22 antibody is human, mouse, and rat reactive. Multiple isoforms of IL-22 are known to exist.
Purity/Purification
IL-22 antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
179
Applicable Applications for anti-IL22 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
IL-22 antibody can be used for detection of IL-22 by Western blot at 1 - 2 mug/ml. Antibody can also be used for Immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
IL-22 antibody was raised against a 17 amino acid peptide near the center of human IL-22
Buffer
IL-22 antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
IL-22 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
Western Blot (WB)
(Western blot analysis of IL-22 in HeLa cell lysate with IL-22 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide. )
Western Blot (WB)
(Western blot analysis of IL-22 in HeLa cell lysate with IL-22 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide. )
Immunocytochemistry (IHC)
(Immunocytochemistry of IL-22 in HeLa cells with IL-22 antibody at 5 μg/mL.)
Immunocytochemistry (IHC)
(Immunocytochemistry of IL-22 in HeLa cells with IL-22 antibody at 5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of IL-22 in HeLa cells with IL-22 antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of IL-22 in HeLa cells with IL-22 antibody at 20 μg/mL.)
Related Product Information for anti-IL22 antibody
Interleukin-22 (IL-22) is a cytokine important for the modulation of tissue responses during inflammation (1). Unlike the distantly related IL-10, IL-22 does not inhibit the production of proinflammatory cytokines in monocytes in response to LPS, but it has some inhibitory effects on IL-4 production from Th2 T cells. IL-22 is expressed by both the adaptive arm of the immune system such as CD4 T cell subsets including Th17 cells, as well as by innate lymphocytes such as NK and LTi-like cells (2). IL-22 is highly expressed in several chronic inflammatory conditions, and studies suggest that IL-22 plays both inflammatory and protective roles (3).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted: 20 kDa
Observed: 25 kDa
Observed: 25 kDa
NCBI Official Full Name
interleukin-22
NCBI Official Synonym Full Names
interleukin 22
NCBI Official Symbol
IL22
NCBI Official Synonym Symbols
TIFa; IL-21; IL-22; ILTIF; IL-TIF; IL-D110; zcyto18; TIFIL-23
NCBI Protein Information
interleukin-22; cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL-10-related T-cell-derived-inducible factor
UniProt Protein Name
Interleukin-22
Protein Family
UniProt Gene Name
IL22
UniProt Synonym Gene Names
ILTIF; ZCYTO18; IL-22; IL-TIF
UniProt Entry Name
IL22_HUMAN
Uniprot Description
IL22: Cytokine that contributes to the inflammatory response in vivo. Belongs to the IL-10 family.
Protein type: Cytokine; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 12q15
Cellular Component: extracellular space; extracellular region
Molecular Function: cytokine activity; interleukin-22 receptor binding
Biological Process: cell-cell signaling; response to glucocorticoid stimulus; acute-phase response; inflammatory response
Research Articles on IL22
Similar Products
Product Notes
The IL22 il22 (Catalog #AAA153464) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IL-22 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's IL-22 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). IL-22 antibody can be used for detection of IL-22 by Western blot at 1 - 2 mug/ml. Antibody can also be used for Immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the IL22 il22 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-22, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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