Rabbit Hexokinase 1 Polyclonal Antibody | anti-HK1 antibody

Hexokinase 1 Antibody

Cat. Num. AAA154388

• Gene Names: HK1; HKD; HKI; HXK1; HMSNR; HK1-ta; HK1-tb; HK1-tc
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity chromatography purified via peptide column.
• Synonyms: Hexokinase 1; Polyclonal Antibody; Hexokinase 1 Antibody; HK; HXK; anti-HK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Hexokinase 1 antibody is human, mouse and rat reactive. Multiple isoforms of Hexokinase 1 are known to exist.
• Purity/Purification: Affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 917

• Applicable Applications for anti-HK1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Hexokinase 1 antibody can be used for detection of Hexokinase 1 by Western blot at 1 - 2 mug/ml. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Immunogen: Hexokinase 1 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human Hexokinase 1.
• Buffer: Supplied in PBS containing 0.02% sodium azide.
• Positive Control 1: Rat Bladder Tissue Lysate
• Conjugate: Unconjugated
• Preparation and Storage: Store at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of Hexokinase 1 in rat bladder tissue lysate with Hexokinase 1 antibody at (A) 1 and (B) 2 μg/ml.)

Immunohistochemistry (IHC)

(Immunohistochemistry of Hexokinase 1 in rat stomach tissue with Hexokinase 1 antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of Hexokinase 1 in rat stomach tissue with Hexokinase 1 antibody at 20 μg/mL.)

Related Product Information for anti-HK1 antibody

There are four major glucose-phosphorylating isoenzymes, designated Hexokinase 1 I, II, III, and IV (1). Hexokinase 1 activity is involved in the first step in several metabolic pathways including phosphorylation of glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway (1,2). Hexokinase 1 2 is the predominant Hexokinase 1 isozyme expressed in insulin-responsive tissues such as skeletal muscle and its expression is insulin-responsive (3). It is involved in the increased rate of glycolysis seen in rapidly growing cancer cells (4).

Product Categories/Family for anti-HK1 antibody

Homeostasis

References

Chen T, Ning D, Sun H, et al. Sequence Analysis and Molecular Characterization of Clonorchis sinensis Hexokinase 1, an Unusual Trimeric 50-kDa Glucose-6-Phosphate-Sensitive Allosteric Enzyme. PLoS One 2014; 9:e107940. Halestrap AP, Pereira GC, and Pasdois P. The role of Hexokinase 1 in cardioprotection-mechanism and potential for translation. Br. J. Pharmacol. 2014; epub. Roberts DJ and Miyamoto S. Hexokinase 1 II integrates energy metabolism and cellular protection: Akting on mitochondria and TORCing to autophagy. Cell Death Differ. 2014; epub. Wang L, Xiong H, Wu F, et al. Hexokinase 1 2-Mediated Warburg Effect Is Required for PTEN- and p53-Deficiency-Driven Prostate Cancer Growth. Cell Rep. 2014; 8:1461-74.

NCBI and Uniprot Product Information

• NCBI GI #: 188497754
• NCBI GeneID: 3098
• NCBI Accession #: NP_000179
• NCBI GenBank Nucleotide #: NM_000188.2
• UniProt Accession #: P19367; O43443; O43444; O75574; Q5VTC3; Q96HC8; Q9NNZ4; Q9NNZ5; E9PCK0
• Molecular Weight: Predicted: 101 kDa; Observed: 103 kDa
• NCBI Official Full Name: hexokinase-1 isoform HKI
• NCBI Official Synonym Full Names: hexokinase 1
• NCBI Official Symbol: HK1
• NCBI Official Synonym Symbols: HKD; HKI; HXK1; HMSNR; HK1-ta; HK1-tb; HK1-tc
• NCBI Protein Information: hexokinase-1
• UniProt Protein Name: Hexokinase-1
• Protein Family: Probable histidine kinase
• UniProt Gene Name: HK1
• UniProt Synonym Gene Names: HK I
• UniProt Entry Name: HXK1_HUMAN

NCBI Description

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific. [provided by RefSeq, Apr 2016]