Western Blot (WB)
(Anti-STEAP1 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:40 kDa Observed band size:45 kDa)
Rabbit anti-Human STEAP1 Polyclonal Antibody | anti-STEAP1 antibody
Anti-STEAP1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
STEAP1; Polyclonal Antibody; Anti-STEAP1 Antibody; Rabbit Polyclonal; STEAP1 Antibody; Rabbit PAb; Antigen Affinity Purified; six transmembrane epithelial antigen of the prostate 1; Anti-PRSS24 Antibody; Anti-STEAP Antibody; anti-STEAP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human STEAP1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-STEAP1 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IP: 1-5uL/mg of lysate
IP: 1-5uL/mg of lysate
Immunogen
E Coli-derived Human STEAP1 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human STEAP1 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-STEAP1 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:40 kDa Observed band size:45 kDa)
Western Blot (WB)
(Anti-STEAP1 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:40 kDa Observed band size:45 kDa)
Immunoprecipitation (IP)
(STEAP1 was immunoprecipitated using: Lane A:0.5 mg A431 Whole Cell Lysate 4 uL anti-STEAP1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-STEAP1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa Observed band size :47 kDa)
Immunoprecipitation (IP)
(STEAP1 was immunoprecipitated using: Lane A:0.5 mg A431 Whole Cell Lysate 4 uL anti-STEAP1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-STEAP1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa Observed band size :47 kDa)
Related Product Information for anti-STEAP1 antibody
The six-transmembrane epithelial antigen of the prostate 1 (STEAP1), a member of the STEAP family, is a general tumor antigen that functions as a potential channel or transporter protein. It is overexpressed in certain cancers and is viewed as a promising therapeutic target. STEAP1 is a transmembrane protein of epithelial cells, mostly located at cell-cell junctions, and is overexpressed in several types of tumors, particularly prostate cancer. STEAP1 is located in the plasma membrane of epithelial cells and may play an important role in inter-and intracellular communication. Several studies suggest STEAP1 as a potential biomarker and an immunotherapeutic target for prostate cancer. STEAP1 is a cell surface antigen overexpressed in multiple cancers and is associated with malignancy and disease prognosis.
Product Categories/Family for anti-STEAP1 antibody
References
Xie J, et al. (2019) Steap1 inhibits breast cancer metastasis and is associated with epithelial-mesenchymal transition procession. Clin Breast Cancer 19 (1): e195-e207.Lee CH, et al. (2016) The prognostic role of steap1 expression determined via immunohistochemistry staining in predicting prognosis of primary colorectal cancer: A survival analysis. Int J Mol Sci 17 (4): 592.Gomes IM, et al. (2018) Knockdown of steap1 inhibits cell growth and induces apoptosis in lncap prostate cancer cells counteracting the effect of androgens. Med Oncol 35 (3): 40.Gomes IM, et al. (2014) Steap1 is overexpressed in prostate cancer and prostatic intraepithelial neoplasia lesions, and it is positively associated with gleason score. Urol Oncol 32 (1): 53.e23-59.
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Product Notes
The STEAP1 (Catalog #AAA8108994) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-STEAP1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's STEAP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:2000 IP: 1-5uL/mg of lysate. Researchers should empirically determine the suitability of the STEAP1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STEAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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