Western Blot (WB)
(Anti-HNF4G rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse stomach tissue lysate Lane B: Mouse spleen tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:46 kDa Observed band size:45 kDa)
Rabbit anti-Mouse HNF-4 gamma/HNF4G Polyclonal Antibody | anti-HNF4G antibody
Anti-HNF-4 gamma/HNF4G Antibody, Rabbit Polyclonal
Reactivity
Mouse
Applications
Western Blot, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
HNF-4 gamma/HNF4G; Polyclonal Antibody; Anti-HNF-4 gamma/HNF4G Antibody; Rabbit Polyclonal; HNF-4 gamma Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-Nr2a2 Antibody; anti-HNF4G antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse HNF-4 gamma/HNF4G
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-HNF4G antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IP: 1-5uL/mg of lysate
IP: 1-5uL/mg of lysate
Immunogen
A synthetic peptide corresponding to the N-terminus of the Mouse HNF-4 gamma
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Mouse HNF-4 gamma, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-HNF4G rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse stomach tissue lysate Lane B: Mouse spleen tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:46 kDa Observed band size:45 kDa)
Western Blot (WB)
(Anti-HNF4G rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse stomach tissue lysate Lane B: Mouse spleen tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:46 kDa Observed band size:45 kDa)
Immunoprecipitation (IP)
(FGF5 was immunoprecipitated using: Lane A:0.5 mg mousespleen tissue Lysate 4 uL anti-FGF5 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-FGF5 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 46 kDa Observed band size :46 kDa)
Immunoprecipitation (IP)
(FGF5 was immunoprecipitated using: Lane A:0.5 mg mousespleen tissue Lysate 4 uL anti-FGF5 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-FGF5 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 46 kDa Observed band size :46 kDa)
Related Product Information for anti-HNF4G antibody
The hepatocyte nuclear factor 4 gamma (HNF4G), a member of orphan nuclear receptors, is up-regulated and functions as an oncoprotein in bladder cancer. miR-34a is a member of the miR-34 family and acts as a tumor suppressor in bladder cancer. miR-34a-HNF4G axis is an important pathway modulating cell viability, proliferation, and invasion of bladder cancer cells. HNF4G expression was elevated in lung cancer tissues as compared to adjacent normal lung tissues, that HNF4G exerts an oncogenic role in lung cancer by promoting cell proliferation and that HNF4G expression is a potential prognosis factor for lung cancer.
NCBI and Uniprot Product Information
UniProt Accession #
Protein Family
Similar Products
Product Notes
The HNF4G (Catalog #AAA8101083) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HNF-4 gamma/HNF4G Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HNF-4 gamma/HNF4G can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:2000 IP: 1-5uL/mg of lysate. Researchers should empirically determine the suitability of the HNF4G for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HNF-4 gamma/HNF4G, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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