Rabbit anti-Human MRPL12 Polyclonal Antibody | anti-MRPL12 antibody

Anti-MRPL12 Antibody, Rabbit Polyclonal

Cat. Num. AAA8108633

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: MRPL12; Polyclonal Antibody; Anti-MRPL12 Antibody; Rabbit Polyclonal; MRPL12 Antibody; Rabbit PAb; Antigen Affinity Purified; mitochondrial ribosomal protein L12; Anti-5c5-2 Antibody; Anti-L12mt Antibody; Anti-MRP-L31/34 Antibody; Anti-MRPL7 Antibody; Anti-MRPL7/L12 Antibody; Anti-RPML12 Antibody; anti-MRPL12 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MRPL12
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-MRPL12 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:100-1:400; ICC/IF: 1:100-1:500; IP: 0.5-2uL/mg of lysate
• Immunogen: E Coli-derived Human MRPL12 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human MRPL12 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-MRPL12 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:21 kDa Observed band size:21 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human MRPL12 in human kidney with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human MRPL12 in human colon with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of MRPL12 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human MRPL12 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

Immunoprecipitation (IP)

(MRPL12 was immunoprecipitated using: Lane A:0.5 mg A431 Whole Cell Lysate 4 uL anti-MRPL12 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-MRPL12 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa Observed band size :21 kDa)

Related Product Information for anti-MRPL12 antibody

Scientists have characterized a new delayed-early response mRNA encoding a 21-kDa product (MRPL12) that accumulates during the G1 phase of growth-stimulated cells. MRPL12 is the mammalian homolog to chloroplastic and bacterial L12 ribosomal proteins. MRPL12 proteins are associated in vitro and cofractionate with ribosomal structures, as is the case for prokaryotic L12 proteins. Expression of a dominant inhibitory truncated protein leads to a severe reduction in cell growth by inhibiting mitochondrial ATP production. MRPL12 is the first mammalian mitochondrial ribosomal protein to be characterized. The human MRPL12 protein is encoded by a unique gene located on chromosome 17 (q25-qter). As no predisposition to colon cancer linked to this chromosomal region was hitherto reported, the MRPL12 gene might be involved in the process of differentiation of colonic epithelial cells.

References

Marty L, et al. (1997) Expression and human chromosomal localization to 17q25 of the growth-regulated gene encoding the mitochondrial ribosomal protein mrpl12. Genomics 41 (3): 453-457.Marty L, et al. (1996) A delayed-early response nuclear gene encoding mrpl12, the mitochondrial homologue to the bacterial translational regulator l7/l12 protein. J Biol Chem 271 (19): 11468-11476.