Rabbit Src Polyclonal Antibody | anti-Src antibody

Src, phosphorylated (Tyr529) (Proto-oncogene Tyrosine-protein Kinase Src, Proto-oncogene c-Src, pp60c-src, p60-Src, SRC1)

Cat. Num. AAA626796

• Gene Names: Src; AW259666; pp60c-src
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Src; Polyclonal Antibody; phosphorylated (Tyr529) (Proto-oncogene Tyrosine-protein Kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src; SRC1); Anti -Src; anti-Src antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Src when phosphorylated at Tyr529. Species Cross-Reactivity: mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-Src antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot:1:500-1:1,000; Immunohistochemistry:1:50-1:100
• Immunogen: Synthetic phosphopeptide corresponding to human Src around the phosphorylation site of tyrosine 529 (P-Q-YP-Q-P).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-Src antibody

Src is a protein tyrosine kinase known to regulate cellular adhesion. Composed of three major domains; SH2, SH3 and a kinase catalytic domain, Src can be switched from an inactive to an active state though control of its phosphorylation state or through protein-protein interaction. Phosphorylation of Y529 by Csk and Chk inactivates Src, while dephosphorylation of Y529 will modify its conformation to an open active state. Mutation of Y529 leads to Scr overactivity. Several cancers including colon and breast cancer have been associated with an increase of Src activity.

Product Categories/Family for anti-Src antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 62635494
• NCBI GeneID: 20779
• UniProt Accession #: P05480; Q2M4I4
• Molecular Weight: 60,645 Da
• NCBI Official Full Name: Src
• NCBI Official Synonym Full Names: Rous sarcoma oncogene
• NCBI Official Symbol: Src
• NCBI Official Synonym Symbols: AW259666; pp60c-src
• NCBI Protein Information: neuronal proto-oncogene tyrosine-protein kinase Src; p60-Src; proto-oncogene c-Src
• UniProt Protein Name: Neuronal proto-oncogene tyrosine-protein kinase Src
• Protein Family: Src-like-adapter
• UniProt Gene Name: Src
• UniProt Synonym Gene Names: p60-Src
• UniProt Entry Name: SRC_MOUSE

Uniprot Description

Src: proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme. Two alternatively spliced isoforms have been described.

Protein type: Kinase, protein; Oncoprotein; Protein kinase, TK; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); TK group; Src family

Cellular Component: extrinsic to internal side of plasma membrane; cytoskeleton; membrane; mitochondrion; perinuclear region of cytoplasm; mitochondrial inner membrane; cytoplasm; plasma membrane; actin filament; intracellular; caveola; nucleus

Molecular Function: transferase activity; protein domain specific binding; protein binding; ephrin receptor binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; non-membrane spanning protein tyrosine kinase activity; kinase activity; ATP binding; hormone receptor binding; protein kinase activity

Biological Process: epidermal growth factor receptor signaling pathway; oogenesis; regulation of estrogen receptor signaling pathway; cell migration; progesterone receptor signaling pathway; peptidyl-tyrosine phosphorylation; immune system process; regulation of cell cycle; platelet-derived growth factor receptor signaling pathway; cell cycle; uterus development; protein amino acid phosphorylation; regulation of cell proliferation; regulation of protein binding; forebrain development; innate immune response; cell adhesion; phosphorylation; bone resorption