Testing Data
(Lysates from untransfected (Lane 1) and Src transfected (lanes 2 and 3) CEF cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with MBS627770 for 2 hours at RT. Lane 3 was pre-incubated with the blocking peptide immunogen corresponding to phospho-Src (Tyr418). Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-Src.)
Rabbit Src Polyclonal Antibody | anti-Src antibody
Src, phosphorylated (Tyr418) (pp60Src, pp60c-Src)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: Western Blot: 1:500-1:2000. Detects phospho-Src (Tyr418) in RIPA lysate of E16 rat heart.
Optimal dilution to be determined by researcher.
Testing Data
(Lysates from untransfected (Lane 1) and Src transfected (lanes 2 and 3) CEF cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with MBS627770 for 2 hours at RT. Lane 3 was pre-incubated with the blocking peptide immunogen corresponding to phospho-Src (Tyr418). Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-Src.)
Testing Data
(Lysates from untransfected (Lane 1) and Src transfected (lanes 2 and 3) CEF cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with MBS627770 for 2 hours at RT. Lane 3 was pre-incubated with the blocking peptide immunogen corresponding to phospho-Src (Tyr418). Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-Src.)
NCBI and Uniprot Product Information
Uniprot Description
Src: proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme. Two alternatively spliced isoforms have been described.
Protein type: Protein kinase, tyrosine (non-receptor); Protein kinase, TK; EC 2.7.10.2; Oncoprotein; Kinase, protein; TK group; Src family
Cellular Component: extrinsic to internal side of plasma membrane; cytoskeleton; membrane; mitochondrion; perinuclear region of cytoplasm; mitochondrial inner membrane; cytoplasm; plasma membrane; actin filament; intracellular; caveola; nucleus
Molecular Function: transferase activity; protein domain specific binding; protein binding; ephrin receptor binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; non-membrane spanning protein tyrosine kinase activity; kinase activity; ATP binding; hormone receptor binding; protein kinase activity
Biological Process: oogenesis; epidermal growth factor receptor signaling pathway; regulation of estrogen receptor signaling pathway; cell migration; peptidyl-tyrosine phosphorylation; progesterone receptor signaling pathway; immune system process; regulation of cell cycle; platelet-derived growth factor receptor signaling pathway; cell cycle; uterus development; protein amino acid phosphorylation; regulation of cell proliferation; regulation of protein binding; forebrain development; innate immune response; cell adhesion; phosphorylation; bone resorption
Research Articles on Src
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Product Notes
The Src src (Catalog #AAA627770) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Src, phosphorylated (Tyr418) (pp60Src, pp60c-Src) reacts with Chicken, Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Src can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Suitable for use in Western Blot, Immunocytochemistry, and Immunohistochemistry. Dilution: Western Blot: 1:500-1:2000. Detects phospho-Src (Tyr418) in RIPA lysate of E16 rat heart. Optimal dilution to be determined by researcher. Researchers should empirically determine the suitability of the Src src for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Src, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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