Rabbit anti-Mouse MXI1 Polyclonal Antibody | anti-MXI1 antibody

MXI1, ID (MXI1, BHLHC11, Max-interacting protein 1, Class C basic helix-loop-helix protein 11) (Biotin)

Cat. Num. AAA6323101

• Gene Names: MXI1; MXI; MAD2; MXD2; bHLHc11
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MXI1; Polyclonal Antibody; ID (MXI1; BHLHC11; Max-interacting protein 1; Class C basic helix-loop-helix protein 11) (Biotin); Class C basic helix-loop-helix protein 11; anti-MXI1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MXI1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MXI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-82 amino acids from the Central region of human MXI1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MXI1 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the MXI1 antibody detected the MXI1 protein (arrow).)

Related Product Information for anti-MXI1 antibody

Expression of the c-myc gene, which produces an oncogenic transcription factor, is tightly regulated in normal cells but is frequently deregulated in human cancers. The protein encoded by this gene is a transcriptional repressor thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in this gene are frequently found in patients with prostate tumors. Three alternatively spliced transcripts encoding different isoforms have been described. Additional alternatively spliced transcripts may exist but the products of these transcripts have not been verified experimentally.

Product Categories/Family for anti-MXI1 antibody

Antibodies; Transcription Factors

References

Lofstedt, T., et al. Exp. Cell Res. 315(11):1924-1936(2009). Baranzini, S.E., et al. Hum. Mol. Genet. 18(4):767-778(2009). Tsao, C.C., et al. Cancer Biol. Ther. 7(10):1619-1627(2008). Suo, X.H., et al. Zhonghua Xue Ye Xue Za Zhi 28(11):745-749(2007). Dugast-Darzacq, C., et al. FEBS J. 274(17):4643-4653(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 57242782
• NCBI GeneID: 4601
• NCBI Accession #: NP_005953.4; NP_569157.2; NP_001008541.1
• NCBI GenBank Nucleotide #: NM_005962.4
• UniProt Accession #: P50539; Q15887; Q6FHW2; Q96E53; B1ANN7; D3DR25; D3DRA9
• Molecular Weight: 26,062 Da
• NCBI Official Full Name: max-interacting protein 1 isoform a
• NCBI Official Synonym Full Names: MAX interactor 1, dimerization protein
• NCBI Official Symbol: MXI1
• NCBI Official Synonym Symbols: MXI; MAD2; MXD2; bHLHc11
• NCBI Protein Information: max-interacting protein 1; MAX dimerization protein 2; Max-related transcription factor; class C basic helix-loop-helix protein 11
• UniProt Protein Name: Max-interacting protein 1
• Protein Family: Max-interacting protein
• UniProt Gene Name: MXI1
• UniProt Synonym Gene Names: BHLHC11; Max interactor 1; bHLHc11
• UniProt Entry Name: MXI1_HUMAN

NCBI Description

Expression of the c-myc gene, which produces an oncogenic transcription factor, is tightly regulated in normal cells but is frequently deregulated in human cancers. The protein encoded by this gene is a transcriptional repressor thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in this gene are frequently found in patients with prostate tumors. Three alternatively spliced transcripts encoding different isoforms have been described. Additional alternatively spliced transcripts may exist but the products of these transcripts have not been verified experimentally. [provided by RefSeq, Jul 2008]