SRC Kinase / Proto-oncogene c-Src Recombinant Protein | SRC recombinant protein

Recombinant Mouse SRC Kinase / Proto-oncogene c-Src Protein (His & GST tag)

Cat. Num. AAA2546611

• Gene Names: Src; AW259666; pp60c-src
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: SRC Kinase / Proto-oncogene c-Src; Recombinant Mouse SRC Kinase / Proto-oncogene c-Src Protein (His & GST tag); RP23-169M4.1; AW259666; pp60c-src; SRC recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Supplied as sterile 20mM Tris, 500mM NaCl, 10% gly, pH 8.0
• Sequence Length: 535

• Application Notes: The recombinant mouse SRC/GST chimera consists of 773 amino acids and has a calculated molecular mass of 87.7 kDa. It migrates as an approximately 80 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for SRC recombinant protein

Background: Proto-oncogene tyrosine-protein kinase SRC is a hydrophobic protein belonging to the SRC family kinase including nine members that is a family of non-receptor tyrosine kinases. SRC protein may exist in different forms: C-SRC and V-SRC. C-SRC is only activated under certain circumstances where it is required such as growth factor signaling, while V-SRC is a constitutively active as opposed to normal SRC (C-SRC). Thus, V-SRC is an instructive example of an oncogene protein kinase whereas C-SRC is a proto-oncogene protein kinase. Inhibition of SRC with NR2A tyrosine phosphorylation mediated by PSD-95 may contribute to the lithium-induced downregulation of NMDA receptor function and provide neuroprotection against excitotoxicity.

Description: A DNA sequence encoding the mouse SRC (NP_001020566.111) (Met 1-Leu 535) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 70794809
• NCBI GeneID: 20779
• NCBI Accession #: NP_001020566.1
• NCBI GenBank Nucleotide #: NM_001025395.2
• UniProt Accession #: P05480; Q2M4I4
• Molecular Weight: 60,645 Da
• NCBI Official Full Name: neuronal proto-oncogene tyrosine-protein kinase Src isoform 2
• NCBI Official Synonym Full Names: Rous sarcoma oncogene
• NCBI Official Symbol: Src
• NCBI Official Synonym Symbols: AW259666; pp60c-src
• NCBI Protein Information: neuronal proto-oncogene tyrosine-protein kinase Src
• UniProt Protein Name: Neuronal proto-oncogene tyrosine-protein kinase Src
• Protein Family: Proto-oncogene tyrosine-protein kinase
• UniProt Gene Name: Src
• UniProt Synonym Gene Names: p60-Src

Uniprot Description

Src: proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme. Two alternatively spliced isoforms have been described.

Protein type: EC 2.7.10.2; Kinase, protein; Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); Src family; TK group

Chromosomal Location of Human Ortholog: 2 H1|2 78.35 cM

Cellular Component: actin filament; caveola; cell-cell adherens junction; cytoplasm; cytosol; extrinsic to internal side of plasma membrane; late endosome; lysosome; mitochondrial inner membrane; mitochondrion; neuron projection; nucleus; perinuclear region of cytoplasm; plasma membrane; podosome; postsynaptic density

Molecular Function: cell adhesion molecule binding; enzyme binding; ephrin receptor binding; estrogen receptor binding; heme binding; hormone receptor binding; insulin receptor binding; kinase activity; kinase binding; non-membrane spanning protein tyrosine kinase activity; phosphoprotein binding; protein binding; protein C-terminus binding; protein complex binding; protein domain specific binding; protein kinase activity; protein kinase binding; protein kinase C binding; protein-tyrosine kinase activity; receptor binding; SH2 domain binding

Biological Process: activation of protein kinase B; bone resorption; cell migration; cell proliferation; epidermal growth factor receptor signaling pathway; forebrain development; innate immune response; integrin-mediated signaling pathway; negative regulation of apoptosis; negative regulation of caspase activity; negative regulation of focal adhesion formation; negative regulation of mitochondrial depolarization; negative regulation of protein homooligomerization; negative regulation of telomerase activity; negative regulation of telomere maintenance via telomerase; negative regulation of transcription, DNA-dependent; nerve growth factor receptor signaling pathway; oogenesis; peptidyl-serine phosphorylation; peptidyl-tyrosine phosphorylation; phosphorylation; platelet-derived growth factor receptor signaling pathway; positive regulation of apoptosis; positive regulation of cell adhesion; positive regulation of cyclin-dependent protein kinase activity; positive regulation of cytokine secretion; positive regulation of insulin receptor signaling pathway; positive regulation of MAP kinase activity; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of protein amino acid autophosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of protein transport; positive regulation of small GTPase mediated signal transduction; positive regulation of smooth muscle cell migration; positive regulation of transcription, DNA-dependent; progesterone receptor signaling pathway; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein destabilization; regulation of cell cycle; regulation of cell proliferation; regulation of cell-cell adhesion; regulation of estrogen receptor signaling pathway; regulation of protein binding; stress fiber formation; transcytosis; transforming growth factor beta receptor signaling pathway; uterus development