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Western Blot (Western Blot analysis using SRC antibodyWestern Blot showing SRC antibody used against truncated SRC-His recombinant protein (1) and PMA treated THP-1 cell lysate (2).)

Mouse SRC Monoclonal Antibody | anti-SRC antibody

SRC antibody

Gene Names
Src; AW259666; pp60c-src
Applications
ELISA, Western Blot
Synonyms
SRC; Monoclonal Antibody; SRC antibody; Monoclonal SRC; Anti-SRC; Schmidt Ruppin A 2; V src sarcoma; Anti SRC; anti-SRC antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
5D10C4
Form/Format
Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
541
Applicable Applications for anti-SRC antibody
ELISA (EIA), Western Blot (WB)
Application Notes
ELISA: 1:10,000
WB: 1:500-1:2000
Biological Significance
SRC may play a role in the regulation of embryonic development and cell growth. The SRC protein is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Cross-Reactivity
Human
Immunogen
SRC antibody was raised in Mouse using a purified recombinant fragment of SRC(aa10-193) expressed in E Coli as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot

(Western Blot analysis using SRC antibodyWestern Blot showing SRC antibody used against truncated SRC-His recombinant protein (1) and PMA treated THP-1 cell lysate (2).)

Western Blot (Western Blot analysis using SRC antibodyWestern Blot showing SRC antibody used against truncated SRC-His recombinant protein (1) and PMA treated THP-1 cell lysate (2).)
Related Product Information for anti-SRC antibody
Mouse monoclonal SRC antibody
Product Categories/Family for anti-SRC antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
60,645 Da
NCBI Official Full Name
Src
NCBI Official Synonym Full Names
Rous sarcoma oncogene
NCBI Official Symbol
Src
NCBI Official Synonym Symbols
AW259666; pp60c-src
NCBI Protein Information
neuronal proto-oncogene tyrosine-protein kinase Src
UniProt Protein Name
Neuronal proto-oncogene tyrosine-protein kinase Src
Protein Family
UniProt Gene Name
Src
UniProt Synonym Gene Names
p60-Src
UniProt Entry Name
SRC_MOUSE

Uniprot Description

Src: proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme. Two alternatively spliced isoforms have been described.

Protein type: Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Kinase, protein; Oncoprotein; Protein kinase, TK; TK group; Src family

Cellular Component: extrinsic to internal side of plasma membrane; cytoskeleton; membrane; mitochondrion; perinuclear region of cytoplasm; mitochondrial inner membrane; cytoplasm; plasma membrane; actin filament; caveola; intracellular; nucleus

Molecular Function: transferase activity; protein domain specific binding; protein binding; ephrin receptor binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; non-membrane spanning protein tyrosine kinase activity; kinase activity; ATP binding; protein kinase activity; hormone receptor binding

Biological Process: oogenesis; epidermal growth factor receptor signaling pathway; regulation of estrogen receptor signaling pathway; cell migration; peptidyl-tyrosine phosphorylation; progesterone receptor signaling pathway; immune system process; regulation of cell cycle; platelet-derived growth factor receptor signaling pathway; uterus development; cell cycle; protein amino acid phosphorylation; regulation of cell proliferation; regulation of protein binding; forebrain development; innate immune response; cell adhesion; phosphorylation; bone resorption

Research Articles on SRC

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Product Notes

The SRC src (Catalog #AAA830900) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's SRC can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ELISA: 1:10,000 WB: 1:500-1:2000. Researchers should empirically determine the suitability of the SRC src for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SRC, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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