Rabbit Src Polyclonal Antibody | anti-Src antibody

Src, phosphorylated (Tyr527) (pp60Src, pp60c-Src)

Cat. Num. AAA628913

• Gene Names: Src; AW259666; pp60c-src
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Src; Polyclonal Antibody; phosphorylated (Tyr527) (pp60Src; pp60c-Src); Anti -Src; anti-Src antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Src only when phosphorylated at Tyr527. It may crossreact with other Src family members Yes, Fyn, Fgr and Yrk when phosphorylated at the equivalent sites. Species Crossreactivity: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

• Applicable Applications for anti-Src antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1:1000; Immunohistochemistry (Paraffin): 1:50
• Immunogen: Synthetic peptide (KLH coupled) corresponding to residues surrounding Tyr527 of human Src.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from NIH/3T3 cells with MBS628913 (A, B) or v-Src antibody (C, D). The phospho-specificity of the antibody was characterized by treating the membrane without (A, C) or with (B, D) calf intestinal alkaline phosphatase (CIP) after Western transfer.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of paraffin-embedded human breast carcinoma showing membrane and cytoplasmic localization of phosphorylated Src, using MBS628913.)

Related Product Information for anti-Src antibody

The Src family of protein tyrosine kinases is important in the regulation of growth and differentiation of eukaryotic cells (1,2). Src activity is regulated by tyrosine phosphorylation at two sites with opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain upregulates the enzyme; phosphorylation of Tyr527 in the C-terminal tail by Csk renders the enzyme less active (3,4).

Product Categories/Family for anti-Src antibody

Antibodies; Abs to Protein Kinase Substrates

NCBI and Uniprot Product Information

• NCBI GI #: 62635494
• NCBI GeneID: 20779
• UniProt Accession #: P05480; Q2M4I4
• Molecular Weight: 60,645 Da
• NCBI Official Full Name: Src
• NCBI Official Synonym Full Names: Rous sarcoma oncogene
• NCBI Official Symbol: Src
• NCBI Official Synonym Symbols: AW259666; pp60c-src
• NCBI Protein Information: neuronal proto-oncogene tyrosine-protein kinase Src; p60-Src; proto-oncogene c-Src
• UniProt Protein Name: Neuronal proto-oncogene tyrosine-protein kinase Src
• Protein Family: Src-like-adapter
• UniProt Gene Name: Src
• UniProt Synonym Gene Names: p60-Src
• UniProt Entry Name: SRC_MOUSE

Uniprot Description

Src: proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme. Two alternatively spliced isoforms have been described.

Protein type: Oncoprotein; Protein kinase, TK; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Kinase, protein; TK group; Src family

Cellular Component: extrinsic to internal side of plasma membrane; cytoskeleton; membrane; mitochondrion; perinuclear region of cytoplasm; cytoplasm; mitochondrial inner membrane; plasma membrane; actin filament; caveola; intracellular; nucleus

Molecular Function: transferase activity; protein domain specific binding; protein binding; ephrin receptor binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; non-membrane spanning protein tyrosine kinase activity; kinase activity; ATP binding; hormone receptor binding; protein kinase activity

Biological Process: oogenesis; epidermal growth factor receptor signaling pathway; regulation of estrogen receptor signaling pathway; cell migration; progesterone receptor signaling pathway; peptidyl-tyrosine phosphorylation; immune system process; regulation of cell cycle; platelet-derived growth factor receptor signaling pathway; uterus development; cell cycle; protein amino acid phosphorylation; regulation of cell proliferation; regulation of protein binding; forebrain development; innate immune response; cell adhesion; phosphorylation; bone resorption