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Dot Blot (DB) (Dot blot analysis of anti-Phospho-SRC-Y419 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Rabbit anti-Human SRC Polyclonal Antibody | anti-Src antibody

SRC, phosphorylated (Tyr419) (Proto-oncogene Tyrosine-protein Kinase Src, p60-Src, c-Src, Proto-oncogene c-Src, pp60c-src, SRC1)

Gene Names
Src; AW259666; pp60c-src
Reactivity
Human
Applications
ELISA, Gel Super Shift Assay
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
SRC; Polyclonal Antibody; phosphorylated (Tyr419) (Proto-oncogene Tyrosine-protein Kinase Src; p60-Src; c-Src; Proto-oncogene c-Src; pp60c-src; SRC1); Anti -SRC; anti-Src antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human SRC when phosphorylated at Tyr419..
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.14% sodium azide.
Applicable Applications for anti-Src antibody
ELISA (EL/EIA), Gel Shift Assay (GS/EMSA)
Application Notes
Suitable for use in ELISA and Dot Blot.
Dilution: ELISA: 1:1,000
Dot Blot: 1:500
Immunogen
Synthetic phosphopeptide corresponding to amino acid residues surrounding Tyr419 of human SRC (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-Phospho-SRC-Y419 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Dot Blot (DB) (Dot blot analysis of anti-Phospho-SRC-Y419 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Related Product Information for anti-Src antibody
This proto-oncogene may play a role in the regulation of embryonic development and cell growth. This protein is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase.
Product Categories/Family for anti-Src antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
60,645 Da
NCBI Official Full Name
Src
NCBI Official Synonym Full Names
Rous sarcoma oncogene
NCBI Official Symbol
Src
NCBI Official Synonym Symbols
AW259666; pp60c-src
NCBI Protein Information
neuronal proto-oncogene tyrosine-protein kinase Src; p60-Src; proto-oncogene c-Src
UniProt Protein Name
Neuronal proto-oncogene tyrosine-protein kinase Src
Protein Family
UniProt Gene Name
Src
UniProt Synonym Gene Names
p60-Src
UniProt Entry Name
SRC_MOUSE

Uniprot Description

Src: proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme. Two alternatively spliced isoforms have been described.

Protein type: EC 2.7.10.2; Oncoprotein; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Kinase, protein; TK group; Src family

Cellular Component: extrinsic to internal side of plasma membrane; cytoskeleton; membrane; mitochondrion; perinuclear region of cytoplasm; cytoplasm; mitochondrial inner membrane; plasma membrane; actin filament; intracellular; caveola; nucleus

Molecular Function: transferase activity; protein domain specific binding; protein binding; ephrin receptor binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; kinase activity; ATP binding; protein kinase activity; hormone receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; oogenesis; regulation of estrogen receptor signaling pathway; cell migration; peptidyl-tyrosine phosphorylation; progesterone receptor signaling pathway; immune system process; regulation of cell cycle; platelet-derived growth factor receptor signaling pathway; cell cycle; uterus development; protein amino acid phosphorylation; regulation of cell proliferation; regulation of protein binding; forebrain development; innate immune response; cell adhesion; phosphorylation; bone resorption

Research Articles on Src

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Product Notes

The Src src (Catalog #AAA624177) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SRC, phosphorylated (Tyr419) (Proto-oncogene Tyrosine-protein Kinase Src, p60-Src, c-Src, Proto-oncogene c-Src, pp60c-src, SRC1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SRC can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Gel Shift Assay (GS/EMSA). Suitable for use in ELISA and Dot Blot. Dilution: ELISA: 1:1,000 Dot Blot: 1:500. Researchers should empirically determine the suitability of the Src src for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SRC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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