Western Blot (WB)
(Sample Data: Representative Western blot with Anti-SMAD2 (1:1000) using 15ng of GST-tagged human recombinant SMAD1, SMAD2, SMAD3, SAMD4 and SMAD5.)
Rabbit SMAD2 Polyclonal Antibody | anti-SMAD2 antibody
Anti-SMAD2
Reactivity
Western blot human SMAD2SMAD2 from other species may also be detectable.
Applications
Western Blot
Purity
Affinity chromatography
Synonyms
SMAD2; Polyclonal Antibody; Anti-SMAD2; anti-SMAD2 antibody
Host
Rabbit
Reactivity
Western blot human SMAD2
SMAD2 from other species may also be detectable.
SMAD2 from other species may also be detectable.
Clonality
Polyclonal
Specificity
Recognizes the SMAD2 protein
Purity/Purification
Affinity chromatography
Form/Format
PBS, pH7.4, 0.02%NaN3, 50% glycerol
Applicable Applications for anti-SMAD2 antibody
Western Blot (WB)
Application Notes
WB: 21838:22837
Immunogen
Synthetic peptide corresponding to amino acids 218-222 of human SMAD2
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Western Blot (WB)
(Sample Data: Representative Western blot with Anti-SMAD2 (1:1000) using 15ng of GST-tagged human recombinant SMAD1, SMAD2, SMAD3, SAMD4 and SMAD5.)
Western Blot (WB)
(Sample Data: Representative Western blot with Anti-SMAD2 (1:1000) using 15ng of GST-tagged human recombinant SMAD1, SMAD2, SMAD3, SAMD4 and SMAD5.)
Western Blot (WB)
(Sample Data: Representative Western blot with Anti-SMAD2 (1:500) using 20 ug, 40 ug and 65 ug of Jurkat cell lysate.)
Western Blot (WB)
(Sample Data: Representative Western blot with Anti-SMAD2 (1:500) using 20 ug, 40 ug and 65 ug of Jurkat cell lysate.)
Related Product Information for anti-SMAD2 antibody
SMADs are essential intracellular components for the signal transduction of TGFbeta family members. SMAD2 is an intracellular mediator of TGFbeta family and activin type 1 receptor (1). SMAD2 mediate TGFbeta signaling to regulate cell growth and differentiation. SMAD2 is released from cytoplasmic retention by TGFbeta receptor-mediated phosphorylation. The phosphorylated SMAD2 then forms a heterodimeric complex with SMAD4, and this complex translocates from cytoplasm into nucleus. By interacting with DNA-binding proteins, SMAD2 complexes then positively or negatively regulate the transcription of target genes. Inactivating mutations in SMAD2 have been found in various cancers (2).
Product Categories/Family for anti-SMAD2 antibody
References
1. Masayuki, F. et al: Identification and Characterization of Constitutively Active Smad2 Mutants: Evaluation of Formation of Smad Complex and Subcellular Distribution. Molecular Endocrinol. 2000; 14 (10): 1583-1591.
2. Eppert, K. et al: MADR2 maps to 18q21 and encodes a TGF-beta-regulated MAD-related protein that is functionally mutated in colorectal carcinoma. Cell. 1996; 86: 543-552.
2. Eppert, K. et al: MADR2 maps to 18q21 and encodes a TGF-beta-regulated MAD-related protein that is functionally mutated in colorectal carcinoma. Cell. 1996; 86: 543-552.
Similar Products
Product Notes
The SMAD2 (Catalog #AAA516209) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SMAD2 reacts with Western blot human SMAD2 SMAD2 from other species may also be detectable. and may cross-react with other species as described in the data sheet. AAA Biotech's SMAD2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 21838:22837. Researchers should empirically determine the suitability of the SMAD2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMAD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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