Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
TAK1-TAB1 recombinant protein
TAK1-TAB1, Active
Applications
Western Blot
Synonyms
TAK1-TAB1; Active; TAK1: MAP3K7; TGF1a; TAB1: MAP3K7IP1; 3'-Tab1; MGC57664; TAK1-TAB1 recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Applicable Applications for TAK1-TAB1 recombinant protein
Kinase Assay, Western Blot (WB)
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for TAK1-TAB1 recombinant protein
Recombinant human proteins TAK1 (1-303) and TAB1 (437-end), linked by a small peptide (DFGGGGG), were expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The TAK1 gene accession number is, NM_003188; TAB1 is NM_006116.
TAK1 is a serine/threonine protein kinase that mediates signaling by TGFbeta and morphogenetic protein (BMP)(1). In response to IL-1, TAK1 forms a kinase complex with TAB1 and this complex is required for the activation of nuclear factor kappa B (NfkappaB). TAK1 can also activate MAPK8/JNK and MAP2K4/MKK4 and thus play a role in the cell response to environmental stress. Tak1 is essential for thymocyte development and activation and deletion of TAK1 prevents maturation of single-positive thymocytes displaying CD4 or CD8 (2). Thymocytes lacking TAK1 fail to activate NfkappaB and JNK and are prone to apoptosis upon stimulation.
TAK1 is a serine/threonine protein kinase that mediates signaling by TGFbeta and morphogenetic protein (BMP)(1). In response to IL-1, TAK1 forms a kinase complex with TAB1 and this complex is required for the activation of nuclear factor kappa B (NfkappaB). TAK1 can also activate MAPK8/JNK and MAP2K4/MKK4 and thus play a role in the cell response to environmental stress. Tak1 is essential for thymocyte development and activation and deletion of TAK1 prevents maturation of single-positive thymocytes displaying CD4 or CD8 (2). Thymocytes lacking TAK1 fail to activate NfkappaB and JNK and are prone to apoptosis upon stimulation.
Product Categories/Family for TAK1-TAB1 recombinant protein
References
1.Yamaguchi, K. et al: Identification of a member of the MAPKKK family as a potential mediator of TGF-beta signal transduction. Science 270: 2008-2011, 1995.
2.Liu, H.-H. et al: Essential role of TAK1 in thymocyte development and activation. Proc. Nat. Acad. Sci. 103: 11677-11682, 2006.
2.Liu, H.-H. et al: Essential role of TAK1 in thymocyte development and activation. Proc. Nat. Acad. Sci. 103: 11677-11682, 2006.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The TAK1-TAB1 (Catalog #AAA517193) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TAK1-TAB1 can be used in a range of immunoassay formats including, but not limited to, Kinase Assay, Western Blot (WB). Researchers should empirically determine the suitability of the TAK1-TAB1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TAK1-TAB1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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