Western Blot (WB)
(Sample Data: Western blot using Affinity Purified Anti-Phospho-SMAD1 (Ser206) antibody (1:500 dilution) shows detection of endogenous phosphorylated SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) derived cell lines treated with PBS, BMP2 (5 ng/mL), EGF (1 ng/mL), or NaCl for 1 h at 37°C before harvest. Each lane contains approximately 15 ug of lysate.)
Rabbit SMAD1 Polyclonal Antibody | anti-Phospho-SMAD1 antibody
Anti-Phospho-SMAD1 (Ser206)
Reactivity
Human, Mouse, Rat, Dog
Applications
ELISA, Western Blot
Purity
Immunoaffinity chromatography
Synonyms
SMAD1; Polyclonal Antibody; Anti-Phospho-SMAD1 (Ser206); anti-Phospho-SMAD1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the human SMAD1 protein phosphorylated at Serine 206
Purity/Purification
Immunoaffinity chromatography
Form/Format
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH7.2 + 0.01% (w/v) Sodium Azide
Applicable Applications for anti-Phospho-SMAD1 antibody
ELISA (EIA), Western Blot (WB)
Immunogen
The antibody was produced against synthesized peptide corresponding to the region of amino acids containing Ser206 of the SMAD1 protein
Preparation and Storage
1 year at -20 degree C from date of shipment
Western Blot (WB)
(Sample Data: Western blot using Affinity Purified Anti-Phospho-SMAD1 (Ser206) antibody (1:500 dilution) shows detection of endogenous phosphorylated SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) derived cell lines treated with PBS, BMP2 (5 ng/mL), EGF (1 ng/mL), or NaCl for 1 h at 37°C before harvest. Each lane contains approximately 15 ug of lysate.)
Western Blot (WB)
(Sample Data: Western blot using Affinity Purified Anti-Phospho-SMAD1 (Ser206) antibody (1:500 dilution) shows detection of endogenous phosphorylated SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) derived cell lines treated with PBS, BMP2 (5 ng/mL), EGF (1 ng/mL), or NaCl for 1 h at 37°C before harvest. Each lane contains approximately 15 ug of lysate.)
Related Product Information for anti-Phospho-SMAD1 antibody
SMAD1 is a member of the SMAD family which are signal transducers and transcriptional modulators that mediate multiple signaling pathways. The actions of bone morphogenetic proteins (BMPs) are mediated by SMAD1 and SMAD1 can be phosphorylated and activated by the BMP receptor kinase (1). Phosphorylated SMAD1 forms a complex with SMAD4 that is important for its function in the transcription regulation. The SMAD1-SMAD4 complex is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. The formation of a complex between STAT3 and SMAD1, bridged by p300, is involved in the cooperative signaling of LIF and BMP2 and the subsequent induction of astrocytes from neuronal progenitors (2).
Product Categories/Family for anti-Phospho-SMAD1 antibody
References
1. Hoodless, P. A. et al: MADR1, a MAD-related protein that functions in BMP2 signaling pathways. Cell 85: 489-500, 1996.
2. Nakashima, K. et al: Synergistic signaling in fetal brain by STAT3-Smad1 complex bridged by p300. Science 284: 479-482, 1999.
2. Nakashima, K. et al: Synergistic signaling in fetal brain by STAT3-Smad1 complex bridged by p300. Science 284: 479-482, 1999.
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Product Notes
The Phospho-SMAD1 (Catalog #AAA516207) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Phospho-SMAD1 (Ser206) reacts with Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's SMAD1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Researchers should empirically determine the suitability of the Phospho-SMAD1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMAD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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