Immunohistochemistry (IHC)
(Sample Data: Anti-Phospho-SMAD3 (Ser423) was used at 2.5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong nuclear staining in the majority of epidermal keratinocytes at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.)
Rabbit SMAD3 Polyclonal Antibody | anti-Phospho-SMAD3 antibody
Anti-Phospho-SMAD3 (Ser423)
Reactivity
Human, Xenopus laevis, Xenopus tropicalis, zebrafish, rat, mouse, swine, bovine, chicken.
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Immunoaffinity chromatography
Synonyms
SMAD3; Polyclonal Antibody; Anti-Phospho-SMAD3 (Ser423); anti-Phospho-SMAD3 antibody
Host
Rabbit
Reactivity
Human, Xenopus laevis, Xenopus tropicalis, zebrafish, rat, mouse, swine, bovine, chicken.
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the human SMAD3 protein phosphorylated at Serine425 and Serine425
Purity/Purification
Immunoaffinity chromatography
Form/Format
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH7.2 + 0.01% (w/v) Sodium Azide
Applicable Applications for anti-Phospho-SMAD3 antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Immunogen
The antibody was produced against synthesized synthesized peptide corresponding to amino acids 417-425 of human SMAD3 protein.
Preparation and Storage
1 year at -20 degree C from date of shipment
Immunohistochemistry (IHC)
(Sample Data: Anti-Phospho-SMAD3 (Ser423) was used at 2.5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong nuclear staining in the majority of epidermal keratinocytes at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.)
Immunohistochemistry (IHC)
(Sample Data: Anti-Phospho-SMAD3 (Ser423) was used at 2.5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong nuclear staining in the majority of epidermal keratinocytes at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.)
Western Blot (WB)
(Sample Data: Western Blot of Rabbit anti-SMAD3 pS423 pS425 antibody- HEK293 lysate.)
Western Blot (WB)
(Sample Data: Western Blot of Rabbit anti-SMAD3 pS423 pS425 antibody- HEK293 lysate.)
Related Product Information for anti-Phospho-SMAD3 antibody
SMAD3 is a direct mediator of transcriptional activation by the TGFbeta receptor. The activity of SMAD3 is regulated by the TGFbeta receptors, and SMAD3 is phosphorylated and associated with the ligand-bound receptor complex. TGFbeta stimulation leads to phosphorylation and activation of SMAD3, which form a complex with SMAD4 that accumulate in the nucleus and regulate transcription of target genes such as CDK inhibitor (1). SMAD3 containing a C-terminal truncation acts as a dominant-negative inhibitor of the normal TGFbeta response. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2 (2)
Product Categories/Family for anti-Phospho-SMAD3 antibody
References
1. Inman, G. J. et al: Nucleocytoplasmic shuttling of Smads 2, 3, and 4 permits sensing of TGF-beta receptor activity. Molec. Cell 10: 283-294, 2002.
2. Matsuura, I. et al: Cyclin-dependent kinases regulate the antiproliferative function of Smads. Nature 430: 226-231, 2004.
2. Matsuura, I. et al: Cyclin-dependent kinases regulate the antiproliferative function of Smads. Nature 430: 226-231, 2004.
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Product Notes
The Phospho-SMAD3 (Catalog #AAA516211) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Phospho-SMAD3 (Ser423) reacts with Human, Xenopus laevis, Xenopus tropicalis, zebrafish, rat, mouse, swine, bovine, chicken. and may cross-react with other species as described in the data sheet. AAA Biotech's SMAD3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). Researchers should empirically determine the suitability of the Phospho-SMAD3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMAD3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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