Mouse anti-Human IVD Monoclonal Antibody | anti-IVD antibody

Anti-IVD Antibody, Mouse Monoclonal

Cat. Num. AAA8107256

• Gene Names: IVD; ACAD2
• Reactivity: Human
• Applications: Western Blot, ELISA, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A
• Synonyms: IVD; Monoclonal Antibody; Anti-IVD Antibody; Mouse Monoclonal; Isovaleryl-CoA dehydrogenase/IVD Antibody; Mouse MAb; isovaleryl-CoA dehydrogenase; Anti-ACAD2 Antibody; anti-IVD antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone Number: 04
• Specificity: Human Isovaleryl-CoA dehydrogenase/IVD.; No cross-reactivity in ELISA with Insect cell lysate
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-IVD antibody: Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS/FCM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:5000-1:10,000; ICC/IF: 1:100-1:500; FC/FACS/FCM: 1:100-1:500; IP: 0.1-0.5uL/mg of lysate
• Immunogen: Recombinant Human Isovaleryl-CoA dehydrogenase/IVD protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Isovaleryl-CoA dehydrogenase/IVD (rh Isovaleryl-CoA dehydrogenase/IVD). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence staining of Human IVD in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human IVD monoclonal antibody (1:300) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Human IVD expression on HeLa cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human IVD, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

Immunoprecipitation (IP)

(IVD was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg A549 Whole Cell Lysate0.5 uL anti-IVD mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-IVD mouse monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 40 kDaObserved band size: 40 kDa)

Western Blot (WB)

(Anti-IVD mouse monoclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:47 kDaObserved band size:40 kDa)

Related Product Information for anti-IVD antibody

IVD (Isovaleryl-CoA Dehydrogenase) is a Protein Coding gene. IVD is a mitochondrial matrix enzyme that catalyzes the third step in leucine catabolism. IVD plays an essential role in processing proteins obtained from the diet. The body breaks down proteins from food into smaller parts called amino acids. Amino acids can be further processed to provide energy for growth and development. Isovaleryl-CoA dehydrogenase helps process a particular amino acid called leucine. Specifically, isovaleryl-CoA dehydrogenase is responsible for the third step in the breakdown of leucine. This step is a chemical reaction that converts a molecule called isovaleryl-CoA to another molecule, 3-methylcrotonyl-CoA. Additional chemical reactions convert 3-methylcrotonyl-CoA into molecules that are used for energy.

References

BACHHAWAT BK, et al. (1956) Enzymatic carboxylation of beta-hydroxyisovaleryl coenzyme A. J Biol Chem. 219(2):539-50. Ikeda Y, et al. (1983) Purification and characterization of isovaleryl coenzyme A dehydrogenase from rat liver mitochondria. J Biol Chem. 258(2):1077-85. Tanaka K, et al. (1966) Enzymatic carboxylation of beta-hydroxyisovaleryl coenzyme A. J Biol Chem. 219(2):539-50.

NCBI and Uniprot Product Information

• NCBI GI #: 16877964
• NCBI GeneID: 3712
• Molecular Weight: 42,724 Da
• NCBI Official Full Name: Isovaleryl Coenzyme A dehydrogenase
• NCBI Official Synonym Full Names: isovaleryl-CoA dehydrogenase
• NCBI Official Symbol: IVD
• NCBI Official Synonym Symbols: ACAD2
• NCBI Protein Information: isovaleryl-CoA dehydrogenase, mitochondrial; isovaleryl CoA dehydrogenase; isovaleryl Coenzyme A dehydrogenase
• Protein Family: Isovaleryl-CoA dehydrogenase

NCBI Description

Isovaleryl-CoA dehydrogenase (IVD) is a mitochondrial matrix enzyme that catalyzes the third step in leucine catabolism. The genetic deficiency of IVD results in an accumulation of isovaleric acid, which is toxic to the central nervous system and leads to isovaleric acidemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009]