Rabbit anti-Human SKA3 Polyclonal Antibody | anti-SKA3 antibody

Anti-SKA3 Antibody, Rabbit Polyclonal

Cat. Num. AAA8109075

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: SKA3; Polyclonal Antibody; Anti-SKA3 Antibody; Rabbit Polyclonal; SKA3 Antibody; Rabbit PAb; Antigen Affinity Purified; spindle and kinetochore associated complex subunit 3; Anti-C13orf3 Antibody; Anti-RAMA1 Antibody; anti-SKA3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human SKA3
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-SKA3 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:50-1:200; IP: 1-5uL/mg of lysate
• Immunogen: E Coli-derived Human SKA3 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human SKA3 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-SKA3 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:46 kDa Observed band size:56 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human SKA3 in human epididymis with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunoprecipitation (IP)

(SKA3 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 4 uL anti-SKA3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-SKA3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 46 kDa Observed band size :55 kDa)

Related Product Information for anti-SKA3 antibody

The spindle and kinetochore-associated (Ska) protein complex is required for accurate chromosome segregation during mitosis and consists of two copies each of Ska1, Ska2, and Ska3 proteins. The Ska complex contains multiple microtubule-binding elements and promotes kinetochore-microtubule attachment. The Ska1 C-terminal domain (CTD) recruits protein phosphatase 1 (PP1) to kinetochores to promote timely anaphase onset. The Ska complex regulates, and is regulated by, Aurora B. Aurora B phosphorylates both Ska1 and Ska3 to inhibit the kinetochore localization of the Ska complex. Ska3 subunit is required to modulate the microtubule-binding capability of the Ska complex (i) by directly interacting with tubulin monomers and (ii) indirectly by interacting with tubulin contacting regions of Ska1 suggesting an allosteric regulation. Ska3 is essential for accurate timing of late mitosis. Ska3 is linked with cervical cancer.

References

Zhang Q, et al. (2017) Ska3 phosphorylated by cdk1 binds ndc80 and recruits ska to kinetochores to promote mitotic progression. Curr Biol 27 (10): 1477-1484.e1474.Ruan LW, et al. (2020) Ska3 promotes cell growth in breast cancer by inhibiting plk-1 protein degradation. Technol Cancer Res Treat 19 1533033820947488.Hou Y, et al. (2019) Ska3 promotes tumor growth by regulating cdk2/p53 phosphorylation in hepatocellular carcinoma. Cell Death Dis 10 (12): 929.