Rat anti-Human, Mouse Galectin-3 Antibody | anti-Lgals3 antibody

Anti-Galectin-3 Purified

Cat. Num. AAA1802247

• Gene Names: Lgals3; GBP; L-34; gal3; Mac-2
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
• Purity: Purified by protein-A affinity chromatography.
• Synonyms: Galectin-3; Anti-Galectin-3 Purified; Mac-2; GBP; Gal-3; L-34; lectin; galactose binding; soluble 3; anti-Lgals3 antibody

• Host: Rat
• Reactivity: Human, Mouse
• Isotype: Rat IgG2a kappa
• Clone Number: M3/38
• Specificity: The rat monoclonal antibody M3/38 recognizes an epitope within amino acids 40-100 in N-terminal domain of murine galectin 3, a 35kDa lectin expressed both intracellularly, and after its secretion also on the cell surface.
• Purity/Purification: Purified by protein-A affinity chromatography.
• Form/Format: Phosphate buffered saline (PBS), pH7.4, 15mM sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-Lgals3 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)(QC tested), Immunoprecipitation (IP)
• Application Notes: FC/FACS: Recommended dilution: 2-6ug/ml. Extracellular and intracellular staining.
• Immunogen: murine galectin-3
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.

Flow Cytometry (FC/FACS)

(Separation of SK-MEL-30 cells stained using anti-Galectin-3 (M3/38) purified antibody (concentration in sample 2,2ug/ml, DAR APC, red-filled) from SK-MEL-30 cells unstained by primary antibody (DAR APC, black-dashed) in flow cytometry analysis (surface staining) of SK-MEL-30 cell suspension.)

Related Product Information for anti-Lgals3 antibody

Galectin-3 is a galactose-binding lectin, which modulates intercellular interactions and interactions of the cell with ECM, as well as it is a nuclear protein and a component of inner mitochondrial membrane. Galectin-3 binds IgE, and takes part in formation of immunological synapse. It is detected cytoplasmatically in adenomas and carcinomas by immunohistochemistry. It is expressed in colonic and intestinal epithelium, papillary and follicular carcinomas, neoplastic astrocytes, inflammatory macrophages, and some lymphocytes. Upregulation of galectin-3 is involved in cancer progression and metastasis.

References

Fogel S, Guittaut M, Legrand A, Monsigny M, Hébert E: The tat protein of HIV-1 induces galectin-3 expression. Glycobiology. 1999 Apr;9(4):383-7., URL: https://pubmed.ncbi.nlm.nih.gov/10089212/
Sundqvist M, Welin A, Elmwall J, Osla V, Nilsson UJ, Leffler H, Bylund J, Karlsson A: Galectin-3 type-C self-association on neutrophil surfaces; The carbohydrate recognition domain regulates cell function. J Leukoc Biol. 2018 Feb;103(2):341-353., URL: https://www.ncbi.nlm.nih.gov/pubmed/29345346
Nachtigal M, Al-Assaad Z, Mayer EP, Kim K, Monsigny M: Galectin-3 expression in human atherosclerotic lesions. Am J Pathol. 1998 May;152(5):1199-208., URL: https://www.ncbi.nlm.nih.gov/pubmed/9588889,

NCBI and Uniprot Product Information

• NCBI GI #: 126679
• NCBI GeneID: 16854
• UniProt Accession #: P16110
• Molecular Weight: 27,515 Da
• NCBI Official Full Name: Galectin-3
• NCBI Official Synonym Full Names: lectin, galactose binding, soluble 3
• NCBI Official Symbol: Lgals3
• NCBI Official Synonym Symbols: GBP; L-34; gal3; Mac-2
• NCBI Protein Information: galectin-3
• UniProt Protein Name: Galectin-3
• Protein Family: Galectin
• UniProt Gene Name: Lgals3
• UniProt Synonym Gene Names: Gal-3; CBP 35
• UniProt Entry Name: LEG3_MOUSE

Uniprot Description

galectin-3: galactose-specific lectin which binds IgE. Expressed at a high level in the colonic epithelium. Also abundant in activated macrophages.

Protein type: Extracellular matrix; Cell surface; Motility/polarity/chemotaxis

Cellular Component: cell surface; cytoplasm; external side of plasma membrane; extracellular matrix; extracellular region; extracellular space; immunological synapse; membrane; mitochondrial inner membrane; nucleus; proteinaceous extracellular matrix; spliceosome

Molecular Function: advanced glycation end-product receptor activity; carbohydrate binding; chemoattractant activity; disaccharide binding; IgE binding; laminin binding; monosaccharide binding; protein binding

Biological Process: cell differentiation; eosinophil chemotaxis; extracellular matrix organization and biogenesis; immune system process; innate immune response; macrophage chemotaxis; maintenance of protein localization; monocyte chemotaxis; mRNA processing; negative regulation of apoptosis; negative regulation of endocytosis; negative regulation of T cell receptor signaling pathway; neutrophil chemotaxis; positive chemotaxis; positive regulation of angiogenesis; positive regulation of cell proliferation; positive regulation of serotonin secretion; regulation of T cell proliferation; RNA splicing; signal transduction; skeletal development