Mouse SKA3 Monoclonal Antibody | anti-SKA3 antibody

SKA3 Antibody

Cat. Num. AAA9435193

• Gene Names: SKA3; RAMA1; C13orf3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunofluorescence
• Purity: ProA affinity purified
• Synonyms: SKA3; Monoclonal Antibody; SKA3 Antibody; C13orf3 antibody; RAMA1 antibody; SKA3 antibody; SKA3_HUMAN antibody; Spindle and kinetochore associated complex subunit 3 antibody; Spindle and kinetochore-associated protein 3 antibody; anti-SKA3 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone Number: 4G1
• Purity/Purification: ProA affinity purified
• Form/Format: 1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-SKA3 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
• Application Notes: WB: 1:100-1:1000; IP: 1-2ug per 100-500ug of total protein (1 ml of cell lysate)
• Immunogen: Amino acids 154-166 within an internal region of Ska3 of human origin
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Ska3 expression in non-transfected (A) and human Ska3 transfected (B) 293T whole cell lysates.)

Related Product Information for anti-SKA3 antibody

Ska3, also designated C13orf3 or RAMA1, is a 412 amino acid protein that belongs to the RAMA1 family of proteins. A component of the SKA1 complex, Ska3 localizes to the outer kinetochore and spindle microtubules during mito- sis. The SKA1 complex is a microtubule-binding subcomplex of the outer kine- tochore and is composed of two Ska1-Ska2 heterodimers, each heterodimer interacting with a Ska3 homodimer. Within the complex, which is important for chromosome segregation and facilitates microsphere movement along microtubules, Ska3 acts as a mediator of microtubule-stimulated oligomeriza- tion. The gene encoding for Ska3 maps to chromosome 13. Comprising nearly 4% of human DNA, chromosome 13 contains around 114 million base pairs and 400 genes. Key tumor suppressor genes on chromosome 13 include the breast cancer susceptibility gene, BRCA2, and the RB1 (retinoblastoma) gene. RB1 encodes a crucial tumor suppressor protein which, when defective, leads to malignant growth in the retina and has been implicated in a variety of other cancers. The gene SLITRK1, which is associated with Tourette syndrome, is on chromosome 13. As with most chromosomes, polysomy of part or all of chro- mosome 13 is deleterious to development and decreases the odds of survival. Trisomy 13, also known as Patau syndrome, is quite deadly and the few who survive past one year suffer from permanent neurologic defects, difficulty eating and vulnerability to serious respiratory infections.

Product Categories/Family for anti-SKA3 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 260763910
• NCBI GeneID: 221150
• NCBI Accession #: NP_659498
• NCBI GenBank Nucleotide #: NM_145061.5
• UniProt Accession #: Q8IX90; Q5VZV5; Q86WR2; Q8NBG1; Q96D22; A2A330; A2A331; B2RBY2
• Molecular Weight: 43,978 Da
• NCBI Official Full Name: spindle and kinetochore-associated protein 3 isoform 1
• NCBI Official Synonym Full Names: spindle and kinetochore associated complex subunit 3
• NCBI Official Symbol: SKA3
• NCBI Official Synonym Symbols: RAMA1; C13orf3
• NCBI Protein Information: spindle and kinetochore-associated protein 3
• UniProt Protein Name: Spindle and kinetochore-associated protein 3
• Protein Family: Spindle and kinetochore-associated protein
• UniProt Gene Name: SKA3
• UniProt Synonym Gene Names: C13orf3; RAMA1
• UniProt Entry Name: SKA3_HUMAN

NCBI Description

This gene encodes a component of the spindle and kinetochore-associated protein complex that regulates microtubule attachment to the kinetochores during mitosis. The encoded protein localizes to the outer kinetechore and may be required for normal chromosome segregation and cell division. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Uniprot Description

RAMA1: Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the microtubule-stimulated oligomerization. Belongs to the SKA3 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 13q12.11

Cellular Component: spindle microtubule; cytoplasm; outer kinetochore of condensed chromosome

Molecular Function: protein binding

Biological Process: mitosis; cell division; regulation of microtubule polymerization or depolymerization; chromosome segregation