Rabbit SKA3 Polyclonal Antibody | anti-SKA3 antibody

SKA3 Antibody

Cat. Num. AAA151003

• Gene Names: SKA3; RAMA1; C13orf3
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: SKA3 Antibody is affinity chromatography purified via peptide column.
• Synonyms: SKA3; Polyclonal Antibody; SKA3 Antibody; RAMA1; C13orf3; Spindle and kinetochore-associated protein 3; spindle and kinetochore associated complex subunit 3; anti-SKA3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: SKA3 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 412

• Applicable Applications for anti-SKA3 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: SKA3 antibody can be used for detection of SKA3 by Western blot at 0.5 - 1 mug/mL.
• Conjugate: Unconjugated
• Immunogen: SKA3 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human SKA3.
• Buffer: SKA3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: SKA3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of SKA3 in human testis tissue lysate with SKA3 antibody at (A) 0.5 and (B) 1 μg/mL.)

Related Product Information for anti-SKA3 antibody

SKA3 Antibody: Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Like with SKA1 or SKA2, depletion of SKA3 by siRNA delays anaphase transition, resulting in a prolonged a metaphase-like state. These SKA3-depleted cells accumulate high levels of the checkpoint protein Bub1 at kinetochores, suggesting the SKA complex plays a key role in spindle checkpoint silencing and the maintenance of chromosome cohesion in mitosis.

NCBI and Uniprot Product Information

• NCBI GI #: 260763910
• NCBI GeneID: 221150
• NCBI Accession #: NP_659498
• NCBI GenBank Nucleotide #: NM_145061.5
• UniProt Accession #: Q8IX90; Q5VZV5; Q86WR2; Q8NBG1; Q96D22; A2A330; A2A331; B2RBY2
• Molecular Weight: 43,978 Da
• NCBI Official Full Name: spindle and kinetochore-associated protein 3 isoform 1
• NCBI Official Synonym Full Names: spindle and kinetochore associated complex subunit 3
• NCBI Official Symbol: SKA3
• NCBI Official Synonym Symbols: RAMA1; C13orf3
• NCBI Protein Information: spindle and kinetochore-associated protein 3
• UniProt Protein Name: Spindle and kinetochore-associated protein 3
• Protein Family: Spindle and kinetochore-associated protein
• UniProt Gene Name: SKA3
• UniProt Synonym Gene Names: C13orf3; RAMA1
• UniProt Entry Name: SKA3_HUMAN

NCBI Description

This gene encodes a component of the spindle and kinetochore-associated protein complex that regulates microtubule attachment to the kinetochores during mitosis. The encoded protein localizes to the outer kinetechore and may be required for normal chromosome segregation and cell division. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Uniprot Description

RAMA1: Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the microtubule-stimulated oligomerization. Belongs to the SKA3 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 13q12.11

Cellular Component: spindle microtubule; cytoplasm; outer kinetochore of condensed chromosome

Molecular Function: protein binding

Biological Process: mitosis; cell division; regulation of microtubule polymerization or depolymerization; chromosome segregation