Western Blot (WB)
(Western blot analysis of adult mouse brain. The blot was probed with rabbit polyclonal anti-SCAI (N-terminal region) antibody in the presence (lanes 2) or absence (lane 1) of SCAI (N-terminal region) blocking peptide (SX3845), or unrelated peptide (lane 3).)
Rabbit SCAI Polyclonal Antibody | anti-SCAI antibody
SCAI (N-terminal region) Antibody
Gene Names
Scai; AI662729; 9330112M16; A930041I02Rik
Reactivity
Human, Rat, Mouse, Chicken
Applications
Western Blot, ELISA
Synonyms
SCAI; Polyclonal Antibody; SCAI (N-terminal region) Antibody; SCAI (N-terminal region); anti-SCAI antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse, Chicken
Clonality
Polyclonal
Specificity
This antibody was affinity purified using SCAI (N-terminal region) peptide (without carrier). The antibody detects a significant 66kDa* protein corresponding to the molecular mass of SCAI on SDS-PAGE immunoblots of mouse brain and human A431 cells. In brain, the antibody also detects 53 and 41kDa bands.
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-SCAI antibody
Western Blot (WB), ELISA (EIA)
Application Notes
ELISA: 1:2000
WB: 1:1000
WB: 1:1000
Immunogen
SCAI synthetic peptide corresponding to amino acid residues in the N-terminal region of mouse SCAI. This peptide sequence is highly conserved in human, rat, mouse, and chicken SCAI.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Western Blot (WB)
(Western blot analysis of adult mouse brain. The blot was probed with rabbit polyclonal anti-SCAI (N-terminal region) antibody in the presence (lanes 2) or absence (lane 1) of SCAI (N-terminal region) blocking peptide (SX3845), or unrelated peptide (lane 3).)
Western Blot (WB)
(Western blot analysis of adult mouse brain. The blot was probed with rabbit polyclonal anti-SCAI (N-terminal region) antibody in the presence (lanes 2) or absence (lane 1) of SCAI (N-terminal region) blocking peptide (SX3845), or unrelated peptide (lane 3).)
Related Product Information for anti-SCAI antibody
Cellular processes such as proliferation, differentiation, and cell migration are directed through the highly regulated control of transcriptional cofactors. During cell migration, serum response factor (SRF) is a central transcription factor that interacts with MAL to induce gene expression in response to actin assembly changes caused by the RhoA-mDia1 pathway. SCAI (suppressor of cancer cell invasion) is a highly conserved protein that is thought to regulate invasive cell migration and act on the RhoA-mDia1 signal transduction pathway. SCAI localizes in the nucleus, where it is thought to bind and inhibit MAL, by forming a ternary complex with SRF. Suppression of SCAI expression has been shown to upregulate beta1-integrin expression, an intergrin that is also upregulated in several human malignancies. In addition, SCAI is downregulated in various human tumors, and decreased levels of SCAI correlate with increased invasive cell migration. Thus, SCAI is a transcriptional cofactor that may be important for regulating gene expression downstream of mDia1 to direct changes in cell motility.
References
Brandt, D.T. et al. (2009) Cell Cycle. 8(16):2523.
Brandt, D.T. et al. (2009) Nat Cell Biol. 11(5):557.
Brandt, D.T. et al. (2009) Nat Cell Biol. 11(5):557.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,814 Da
NCBI Official Full Name
protein SCAI
NCBI Official Synonym Full Names
suppressor of cancer cell invasion
NCBI Official Symbol
Scai
NCBI Official Synonym Symbols
AI662729; 9330112M16; A930041I02Rik
NCBI Protein Information
protein SCAI
UniProt Protein Name
Protein SCAI
Protein Family
UniProt Gene Name
Scai
UniProt Entry Name
SCAI_MOUSE
Uniprot Description
SCAI: Tumor suppressor which functions to suppress MKL1- induced SRF transcriptional activity. May function in the RHOA- DIAPH1 signal transduction pathway and regulate cell migration through transcriptional regulation of ITGB1. Belongs to the SCAI family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Transcription, coactivator/corepressor
Cellular Component: cytoplasm; integral to membrane; membrane; nuclear membrane; nucleus
Molecular Function: protein binding; transcription corepressor activity
Biological Process: negative regulation of cell migration; negative regulation of Rho protein signal transduction; negative regulation of signal transduction; regulation of transcription, DNA-dependent; transcription, DNA-dependent
Research Articles on SCAI
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Product Notes
The SCAI scai (Catalog #AAA474601) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SCAI (N-terminal region) Antibody reacts with Human, Rat, Mouse, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's SCAI can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). ELISA: 1:2000 WB: 1:1000. Researchers should empirically determine the suitability of the SCAI scai for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SCAI, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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