Rabbit Atrogin-1 Polyclonal Antibody | anti-Fbxo32 antibody

Atrogin-1 Antibody

Cat. Num. AAA474185

• Gene Names: Fbxo32; MAFbx; Gm20361; AI430017; ATROGIN1; 4833442G10Rik
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, ELISA, Immunohistochemistry
• Synonyms: Atrogin-1; Polyclonal Antibody; Atrogin-1 Antibody; Atrogin pAb; anti-Fbxo32 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Specificity: This antibody detects a 41kDa* protein corresponding to the apparent molecular mass of Atrogin on SDS-PAGE immunoblots of mouse muscle tissue lysates. Similar results were seen in C2C12 cell lysate and the band observed in western blot is specifically blocked by Atrogin-1 peptide (AX2045).
• Form/Format: Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-Fbxo32 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: ELISA: 1:2000; IHC: 1:100; WB: 1:1000
• Immunogen: A synthetic peptide (coupled to carrier protein) corresponding to amino acids 23 to 35 in mouse Atrogin. This sequence is highly homologous to human and rat Atrogin (F-box only protein 32).
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot image of mouse gastrocnemius (lanes 1 & 3) and mouse diagphram tissue lysate (lanes 2 & 4). The blot was probed with anti-Atrogin-1 (AP2041; lanes 1-4) in the presence (lanes 3 & 4) or absence (lanes 1 & 2) of Atrogin-1 peptide (AX2045).)

Related Product Information for anti-Fbxo32 antibody

Atrogin-1/Muscle Atrophy F-box (MAFbx) is an E3 ubiquitin ligase that mediates proteolysis events that occur during muscle atrophy. This ATP-dependent ubiquitin-mediated proteolysis occurs in response to a variety of catabolic states in muscle. Atrogin is expressed in heart and skeletal muscle, and is upregulated during muscle atrophy. In addition, Atrogin expression increases in C2C12 myotubes after stimulation with cytokines. Atrogin is thought to recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation during skeletal muscle atrophy. Atrogin interacts with MyoD by ubiquitination via a sequence found in transcriptional coactivators and therefore may play an important role in the course of muscle differentiation by determining the abundance of MyoD. Mice deficient in Atrogin are resistant to muscle atrophy.

References

Bodine, S.C. et al. (2001) Science 294: 1704-8.
Dai, K.S. & Liews, C.C. (2001) J Biol. Chem. 276(26):23992.
Leger, B. et al. (2006) J Physiol. 576(3):923.

NCBI and Uniprot Product Information

• NCBI GI #: 13385848
• NCBI GeneID: 67731
• NCBI Accession #: NP_080622.1
• NCBI GenBank Nucleotide #: NM_026346.3
• UniProt Accession #: Q9CPU7
• Molecular Weight: 41,504 Da
• NCBI Official Full Name: F-box only protein 32
• NCBI Official Synonym Full Names: F-box protein 32
• NCBI Official Symbol: Fbxo32
• NCBI Official Synonym Symbols: MAFbx; Gm20361; AI430017; ATROGIN1; 4833442G10Rik
• NCBI Protein Information: F-box only protein 32
• UniProt Protein Name: F-box only protein 32
• Protein Family: F-box only protein
• UniProt Gene Name: Fbxo32
• UniProt Synonym Gene Names: MAFbx
• UniProt Entry Name: FBX32_MOUSE

Uniprot Description

FBXO32: Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Cellular Component: cytoplasm; nucleolus; Z disc; nucleus

Molecular Function: protein binding

Biological Process: muscle atrophy; response to electrical stimulus involved in regulation of muscle adaptation; response to denervation involved in regulation of muscle adaptation