Rabbit Smad2 Polyclonal Antibody | anti-SMAD2 antibody

Smad2, phosphorylated (Ser245/250/255) (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

Cat. Num. AAA617171

• Gene Names: SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2; MGC22139; MGC34440
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Protein A and peptide affinity chromatography
• Synonyms: Smad2; Polyclonal Antibody; phosphorylated (Ser245/250/255) (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma); Anti -Smad2; anti-SMAD2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Phospho-Smad2 (Ser245/250/255) Detects endogenous levels of Smad2 only when phosphorylated at serines 245, 250 or 255. Species Crossreactivity
• Purity/Purification: Affinity Purified; Protein A and peptide affinity chromatography
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol.

• Applicable Applications for anti-SMAD2 antibody: Western Blot (WB)
• Application Notes: Suitable for use in ELSA and Western Blot.; Dilution: Western Blot: 1:1000
• Immunogen: Synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding serines 245/250/255 of Smad2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot. Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells, mock transfected or transfected with Smad2 or Smad3 and treated with TPA (200 nM) or TGF-beta (100 ng/ml) for 30 minutes, using MBS617171 Smad2 Phosphorylated (Ser245/250/255).)

Western Blot (WB)

(Western blot analysis of extracts from 293 and HeLa cells, untreated or TPA-treated (200 nM for 30 minutes) using MBS617171 Smad2 Phosphorylated (Ser245/250/255) (upper) or Smad2 (lower).)

Related Product Information for anti-SMAD2 antibody

Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-b signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5 and 8, the common-mediator Smad (co-Smad), Smad4, and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Briefly, activated type I receptors associatewith specific R-Smads and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8). Oncogenic Ras antagonizes TGF-b signaling and inhibits the nuclear accumulation of Smad2 and Smad3, which may be explained through MAP kinase dependent phosphorylation of these Smads (9). Cell stimulation with EGF leads to phosphorylation of Smad2 at a cluster of serine-proline sites within its linker region, including Ser245, 250, and 255 (9).

Product Categories/Family for anti-SMAD2 antibody

Antibodies; Abs to Smad Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 2967646
• NCBI GeneID: 4087
• UniProt Accession #: Q15796; Q53XR6; Q7Z5E4
• Molecular Weight: 52,306 Da
• NCBI Official Full Name: Smad2
• NCBI Official Synonym Full Names: SMAD family member 2
• NCBI Official Symbol: SMAD2
• NCBI Official Synonym Symbols: JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2; MGC22139; MGC34440
• NCBI Protein Information: mothers against decapentaplegic homolog 2; SMAD 2; MAD homolog 2; OTTHUMP00000163489; Mad protein homolog; Mad-related protein 2; mother against DPP homolog 2; mothers against DPP homolog 2; Sma- and Mad-related protein 2; SMAD, mothers against DPP homolog 2; MAD, mothers against decapentaplegic homolog 2; Mad, mothers against decapentaplegic homolog 2
• UniProt Protein Name: Mothers against decapentaplegic homolog 2
• Protein Family: Mothers against decapentaplegic
• UniProt Gene Name: SMAD2
• UniProt Synonym Gene Names: MADH2; MADR2
• UniProt Entry Name: SMAD2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]

Uniprot Description

SMAD2: ubiquitously expressed transcription factor phosphorylated and activated by TGF-beta receptor-type kinases. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis. Phosphorylated Smads dimerize with collaborating Smad4 and are translocated into the nucleus, where the transcription of target genes is stimulated. Two alternatively spliced isoforms have been described.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; transcription activator binding; DNA binding; metal ion binding; ubiquitin protein ligase binding; double-stranded DNA binding; chromatin binding; SMAD binding; transcription factor binding; transforming growth factor beta receptor binding; transcription factor activity; phosphatase binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity

Biological Process: developmental growth; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; paraxial mesoderm morphogenesis; gastrulation; primary microRNA processing; palate development; negative regulation of transcription from RNA polymerase II promoter; post-embryonic development; anterior/posterior pattern formation; negative regulation of cell proliferation; regulation of transforming growth factor beta receptor signaling pathway; ureteric bud development; transforming growth factor beta receptor signaling pathway; embryonic foregut morphogenesis; pancreas development; response to glucose stimulus; positive regulation of BMP signaling pathway; pericardium development; transcription initiation from RNA polymerase II promoter; cell fate commitment; transcription, DNA-dependent; regulation of binding; in utero embryonic development; common-partner SMAD protein phosphorylation; embryonic cranial skeleton morphogenesis; SMAD protein complex assembly; organ growth; zygotic determination of dorsal/ventral axis; mesoderm formation; insulin secretion; endoderm formation; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transforming growth factor beta receptor signaling pathway; negative regulation of transcription, DNA-dependent; lung development