Immunofluorescence (IF)
(Immunofluorescence staining of S-Tag in Hela cells, transfected with pSTEP2-Stag-GFERh (Figure A), pSTEP2-his-GFER-Stag (Figure B), pSTEP2-his-Stag-GFER (Figure C), pSTEP2-Stag-GSTT2h (Figure D),pSTEP2-his-Stag-GSTT2 (Figure E) and pSTEP2-his-GSTT2-Stag (Figure F). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-S-Tag polyclonal antibody at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).)
Rabbit S-tag Polyclonal Antibody | anti-S-tag antibody
Anti-S-tag Antibody, Rabbit Polyclonal
Reactivity
S-tag
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
S-tag; Polyclonal Antibody; Anti-S-tag Antibody; Rabbit Polyclonal; Rabbit Pab; Antigen Affinity Purified; anti-S-tag antibody
Host
Rabbit
Reactivity
S-tag
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Recognize N-terminal and C-terminal S tag in fusion proteins
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-S-tag antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:2000-1:10,000
ICC/IF: 1:300-1:10,000
IP: 0.5-2ug/mg of lysate
ICC/IF: 1:300-1:10,000
IP: 0.5-2ug/mg of lysate
Immunogen
A synthetic peptide corresponding to the S-tag sequence (KETAAAKFERQHMDS)
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the S-tag sequence (KETAAAKFERQHMDS), and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of S-Tag in Hela cells, transfected with pSTEP2-Stag-GFERh (Figure A), pSTEP2-his-GFER-Stag (Figure B), pSTEP2-his-Stag-GFER (Figure C), pSTEP2-Stag-GSTT2h (Figure D),pSTEP2-his-Stag-GSTT2 (Figure E) and pSTEP2-his-GSTT2-Stag (Figure F). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-S-Tag polyclonal antibody at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).)
Immunofluorescence (IF)
(Immunofluorescence staining of S-Tag in Hela cells, transfected with pSTEP2-Stag-GFERh (Figure A), pSTEP2-his-GFER-Stag (Figure B), pSTEP2-his-Stag-GFER (Figure C), pSTEP2-Stag-GSTT2h (Figure D),pSTEP2-his-Stag-GSTT2 (Figure E) and pSTEP2-his-GSTT2-Stag (Figure F). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-S-Tag polyclonal antibody at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).)
Immunoprecipitation (IP)
(s-tag was immunoprecipitated using: Lane A:0.5 mg Stag-GFERh transfected 293 Whole Cell Lysate Lane B:0.5 mg his-GFER-Stag(C) transfected 293 Whole Cell Lysate Lane C:0.5 mg his-Stag-GFER(C) transfected 293 Whole Cell Lysate Lane D:0.5 mg Psetp2 transfected 293 Whole Cell Lysate 2 uL anti-s-tag rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-s-tag rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.)
Immunoprecipitation (IP)
(s-tag was immunoprecipitated using: Lane A:0.5 mg Stag-GFERh transfected 293 Whole Cell Lysate Lane B:0.5 mg his-GFER-Stag(C) transfected 293 Whole Cell Lysate Lane C:0.5 mg his-Stag-GFER(C) transfected 293 Whole Cell Lysate Lane D:0.5 mg Psetp2 transfected 293 Whole Cell Lysate 2 uL anti-s-tag rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-s-tag rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.)
Western Blot (WB)
(Anti-S Tag rabbit polyclonal antibody at 1:5000 dilution Lane A: Stag-GFERh(C) transfected 293 cell lysate at 2ug Lane B: his-GFER-Stag(C) transfected 293 cell lysate at 2ug Lane C: his-Stag-GFER(C) transfected 293 cell lysate at 2ug Lane D: Stag-GSTT2h(C) transfected 293 cell lysate at 10ug Lane E: his-Stag-GSTT2(C) transfected 293 cell lysate at 10ug Lane F: his-GSTT2-Stag(C) transfected 293 cell lysate at 10ug Lane G: Nagetive control at 10ug Secondary Dylight 800-labeled Antibody To Rabbit IgG (H+L) at 1/5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions.)
Western Blot (WB)
(Anti-S Tag rabbit polyclonal antibody at 1:5000 dilution Lane A: Stag-GFERh(C) transfected 293 cell lysate at 2ug Lane B: his-GFER-Stag(C) transfected 293 cell lysate at 2ug Lane C: his-Stag-GFER(C) transfected 293 cell lysate at 2ug Lane D: Stag-GSTT2h(C) transfected 293 cell lysate at 10ug Lane E: his-Stag-GSTT2(C) transfected 293 cell lysate at 10ug Lane F: his-GSTT2-Stag(C) transfected 293 cell lysate at 10ug Lane G: Nagetive control at 10ug Secondary Dylight 800-labeled Antibody To Rabbit IgG (H+L) at 1/5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions.)
Related Product Information for anti-S-tag antibody
S-tag is a 15 amino acid truncated form of the S-peptide, KETAAAKFERQHMDS, used in a variety of protein expression applications in mammalian cells. Its small size and excessive solubility have made the S-tag an excellent fusion partner in the production of recombinant proteins. S-tag is the name of an oligopeptide derived from pancreatic ribonuclease A (RNase A). The N-terminus of the original RNase A, also known as S-peptide, consists of 20 amino acid residues, of which only the first 15 are required for ribonuclease activity. This 15 amino acids long peptide is called S15 or S-tag. On DNA-level the S-tag can be attached to the N-or C-terminus of any protein. After gene expression, such a tagged protein can be detected by commercially available antibodies.
References
Park JH, et al. (2001) Detection of pet-vector encoded, recombinant s-tagged proteins using the monoclonal antibody atom-2. Hybridoma 20 (1): 17-23.Murray G, et al. (2001) Calmodulin-binding protein detection using a non-radiolabeled calmodulin fusion protein. Biotechniques 30 (5): 1036-1042.Daskalow K, et al. (2008) Epitope mapping of antibodies against s-tagged fusion proteins and molecular weight markers. Biosci Biotechnol Biochem 72 (2): 346-351.
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Product Notes
The S-tag (Catalog #AAA8101155) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-S-tag Antibody, Rabbit Polyclonal reacts with S-tag and may cross-react with other species as described in the data sheet. AAA Biotech's S-tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:2000-1:10,000 ICC/IF: 1:300-1:10,000 IP: 0.5-2ug/mg of lysate. Researchers should empirically determine the suitability of the S-tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "S-tag, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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