Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse 7-dehydrocholesterol reductase (DHCR7) Standard Curve.)
Mouse 7-Dehydrocholesterol Reductase (DHCR7) ELISA Kit | DHCR7 elisa kit
Mouse 7-Dehydrocholesterol Reductase (DHCR7) ELISA Kit
Gene Names
Dhcr7; AA409147
Reactivity
Mouse
Synonyms
7-Dehydrocholesterol Reductase (DHCR7); Mouse 7-Dehydrocholesterol Reductase (DHCR7) ELISA Kit; DHCR7; SLOS; delta-7-dehydrocholesterol reductase; sterol delta-7-reductase; DHCR7 elisa kit
Reactivity
Mouse
Specificity
Mouse 7-dehydrocholesterol reductase (DHCR7) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DHCR7. No significant cross-reactivity or interference between Mouse DHCR7 and analogues was observed.
Sequence Length
471
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse 7-dehydrocholesterol reductase (DHCR7) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse 7-dehydrocholesterol reductase (DHCR7) Standard Curve.)
Related Product Information for DHCR7 elisa kit
Description: This Mouse 7-dehydrocholesterol reductase (DHCR7) ELISA Kit employs a two-site sandwich ELISA to quantitate DHCR7 in samples. An antibody specific for DHCR7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DHCR7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DHCR7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DHCR7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DHCR7 encodes an enzyme that removes the C(7-8) double bond in the B ring of sterols and catalyzes the conversion of 7-dehydrocholesterol to cholesterol. DHCR7 is ubiquitously expressed and its transmembrane protein localizes to the endoplasmic reticulum membrane and nuclear outer membrane. Mutations in DHCR7 cause Smith-Lemli-Opitz syndrome (SLOS); a syndrome that is metabolically characterized by reduced serum cholesterol levels and elevated serum 7-dehydrocholesterol levels and phenotypically characterized by mental retardation, facial dysmorphism, syndactyly of second and third toes, and holoprosencephaly in severe cases to minimal physical abnormalities and near-normal intelligence in mild cases. Alternative splicing results in multiple transcript variants that encode the same protein.
Background: DHCR7 encodes an enzyme that removes the C(7-8) double bond in the B ring of sterols and catalyzes the conversion of 7-dehydrocholesterol to cholesterol. DHCR7 is ubiquitously expressed and its transmembrane protein localizes to the endoplasmic reticulum membrane and nuclear outer membrane. Mutations in DHCR7 cause Smith-Lemli-Opitz syndrome (SLOS); a syndrome that is metabolically characterized by reduced serum cholesterol levels and elevated serum 7-dehydrocholesterol levels and phenotypically characterized by mental retardation, facial dysmorphism, syndactyly of second and third toes, and holoprosencephaly in severe cases to minimal physical abnormalities and near-normal intelligence in mild cases. Alternative splicing results in multiple transcript variants that encode the same protein.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
7-dehydrocholesterol reductase
NCBI Official Synonym Full Names
7-dehydrocholesterol reductase
NCBI Official Symbol
Dhcr7
NCBI Official Synonym Symbols
AA409147
NCBI Protein Information
7-dehydrocholesterol reductase
UniProt Protein Name
7-dehydrocholesterol reductase
Protein Family
UniProt Gene Name
Dhcr7
UniProt Synonym Gene Names
7-DHC reductase
UniProt Entry Name
DHCR7_MOUSE
Research Articles on DHCR7
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Product Notes
The Mouse DHCR7 dhcr7 (Catalog #AAA9717903) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717903 ELISA Kit recognizes Mouse DHCR7. It is sometimes possible for the material contained within the vial of "7-Dehydrocholesterol Reductase (DHCR7), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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