Western Blot (WB)
(Anti-RUNX3 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: Raji Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:44 kDa)
Rabbit anti-Human RUNX3 Polyclonal Antibody | anti-RUNX3 antibody
Anti-RUNX3 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
RUNX3; Polyclonal Antibody; Anti-RUNX3 Antibody; Rabbit Polyclonal; RUNX3 Antibody; Rabbit PAb; Antigen Affinity Purified; runt related transcription factor 3; Anti-AML2 Antibody; Anti-CBFA3 Antibody; Anti-PEBP2aC Antibody; anti-RUNX3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human RUNX3
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-RUNX3 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:1000
IHC-P: 1:1000-1:5000
IHC-P: 1:1000-1:5000
Immunogen
E Coli-derived Human RUNX3 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human RUNX3 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-RUNX3 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: Raji Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:44 kDa)
Western Blot (WB)
(Anti-RUNX3 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: Raji Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:44 kDa)
Immunohistochemistry (IHC)
(Immunochemical staining of human RUNX3 in human lymph node with rabbit polyclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human RUNX3 in human lymph node with rabbit polyclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-RUNX3 antibody
Runt-related transcription factor 3 (RUNX3) is a member of the Runt domain family that is known to play key roles in various types of tumor. RUNX3, runt-domain transcription factor, is a master regulator of gene expression in major developmental pathways. It acts as a tumor suppressor in many cancers but is oncogenic in certain tumors. It was recently demonstrated that RUNX3 may also be associated with cervical cancer. The transcription factor RUNX3 is a prominent regulator of multiple hematopoietic cell lineages. Gene loss of function studies demonstrated the unique and essential roles of this master regulator in differentiated lymphoid and myeloid cells. As a complementary approach, RUNX3 was upregulated in various leukocyte subsets to probe the instructive role of this 'multilineage'-specific transcription factor.
References
Selvarajan V, et al. (2017) Runx3 is oncogenic in natural killer/t-cell lymphoma and is transcriptionally regulated by myc. Leukemia 31 (10): 2219-2227.Gao QQ, et al. (2020) Transcriptome changes induced by runx3 in cervical cancer cells in vitro. Oncol Lett 19 (1): 651-662.Boto P, et al. (2018) Runx3-mediated immune cell development and maturation. Crit Rev Immunol 38 (1): 63-78.
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Product Notes
The RUNX3 (Catalog #AAA8108241) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RUNX3 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RUNX3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:1000 IHC-P: 1:1000-1:5000. Researchers should empirically determine the suitability of the RUNX3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RUNX3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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