SDS-Page
RuNX3 recombinant protein
RuNX3, 53-186aa, Human, His tag, E Coli
Gene Names
RUNX3; AML2; CBFA3; PEBP2aC
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
RuNX3; 53-186aa; Human; His tag; E Coli; Runt-related transcription factor 3; AML2; CBFA3; PEBP2aC; Runt-related transcription factor 3 Acute myeloid leukemia 2 protein; Acute myeloid leukemia gene 2; AML 2; CBF alpha 3; CBFA 3; Core binding factor alpha 3 subunit; core binding factor; Core binding factor runt domain alpha subunit 3; Core binding factor subunit alpha 3.M574; core-binding factor; FLJ34510; MGC16070; Oncogene AML 2; PEA2 alpha C; PEBP2 alpha C; Pebp2a3; Polyomavirus enhancer binding protein 2 alpha C subunit; runt domain alpha subunit 3; runt related transcription factor 3; RuNX 3; SL3 3 enhancer factor 1 alpha C subunit; SL3/AKV core binding factor alpha C subunit; Transcription factor AML2.; RuNX3 recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol 0.1M NaCl, 1mM DTT
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MRSMVDVLAD HAGELVRTDS PNFLCSVLPS HWRCNKTLPV AFKVVALGDV PDGTVVTVMA GNDENYSAEL RNASAVMKNQ VARFNDLRFV GRSGRGKSFT LTITVFTNPT QVATYHRAIK VTVDGPREPR RHRQK
Sequence Length
415
Applicable Applications for RuNX3 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for RuNX3 recombinant protein
RuNX3, also known as runt-related transcription factor 3, belongs to the RuNX family that regulate the expression of genes involved in cellular differentiation and cell cycle progression. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It is a strong candidate as a gastric cancer tumor suppressor. Recombinant human RuNX3, fused to His-tag at N-terminus, protein was expressed in E Coli and purified by using conventional chromatography techniques
Product Categories/Family for RuNX3 recombinant protein
References
Vogiatzi P. et al. (2006) Cancer Biol Ther. 5 (4):371-4.; Li QL,et al. (2002)Cell. 109 (1):113-24.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17.1 kDa (155aa) confirmed by MALDI-TOF
NCBI Official Full Name
runt-related transcription factor 3 isoform 2
NCBI Official Synonym Full Names
runt related transcription factor 3
NCBI Official Symbol
RUNX3
NCBI Official Synonym Symbols
AML2; CBFA3; PEBP2aC
NCBI Protein Information
runt-related transcription factor 3
UniProt Protein Name
Runt-related transcription factor 3
Protein Family
UniProt Gene Name
RUNX3
UniProt Synonym Gene Names
AML2; CBFA3; PEBP2A3; CBF-alpha-3; PEA2-alpha C; PEBP2-alpha C
NCBI Description
This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
Uniprot Description
CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters. In association with ZFHX3, upregulates CDKN1A promoter activity following TGF-beta stimulation (PubMed:20599712).
Research Articles on RuNX3
Similar Products
Product Notes
The RuNX3 runx3 (Catalog #AAA203402) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's RuNX3 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the RuNX3 runx3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MRSMVDVLAD HAGELVRTDS PNFLCSVLPS HWRCNKTLPV AFKVVALGDV PDGTVVTVMA GNDENYSAEL RNASAVMKNQ VARFNDLRFV GRSGRGKSFT LTITVFTNPT QVATYHRAIK VTVDGPREPR RHRQK. It is sometimes possible for the material contained within the vial of "RuNX3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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