Rabbit anti-Human PAX5 Polyclonal Antibody | anti-PAX5 antibody

Anti-PAX5 Antibody, Rabbit Polyclonal

Cat. Num. AAA8108684

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: PAX5; Polyclonal Antibody; Anti-PAX5 Antibody; Rabbit Polyclonal; PAX5 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-BSAP Antibody; anti-PAX5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PAX5
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-PAX5 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:100-1:400; IP: 5-10uL/mg of lysate
• Immunogen: E Coli-derived Human PAX5 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human PAX5 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-PAX5 rabbit polyclonal antibody at 1:500 dilution Lane A: Daudi Whole Cell Lysate Lane B: Raji Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:42 kDa Observed band size:48 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human PAX5 in human tonsil with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human PAX5 in human lymph node with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

Immunoprecipitation (IP)

(PAX5 was immunoprecipitated using: Lane A:0.5 mg Raji Whole Cell Lysate 4 uL anti-PAX5 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PAX5 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa Observed band size :45 kDa)

Related Product Information for anti-PAX5 antibody

Somatic alterations of the lymphoid transcription factor gene PAX5 (also known as BSAP) are a hallmark of B cell precursor acute lymphoblastic leukemia (B-ALL). PAX5 is a well-known haploinsufficient tumor suppressor gene in human B-cell precursor acute lymphoblastic leukemia (B-ALL) and is involved in various chromosomal translocations that fuse a part of PAX5 with other partners. PAX5, one of nine members of the mammalian paired box (PAX) family of transcription factors, plays an important role in B cell development. Approximately one-third of individuals with pre-B acute lymphoblastic leukemia (ALL) acquire heterozygous inactivating mutations of PAX5 in malignant cells, and heterozygous germline loss-of-function PAX5 mutations cause an autosomal dominant predisposition to ALL. PAX5 is expressed in the immune-privileged brain, B-lymphocytes, lymph nodes, and spleen.

References

Maurya SK, et al. (2018) Co-localization and interaction of pax5 with iba1 in brain of mice. Cell Mol Neurobiol 38 (4): 919-927.
Jamrog L, et al. (2018) Pax5-eln oncoprotein promotes multistep b-cell acute lymphoblastic leukemia in mice. Proc Natl Acad Sci U S A 115 (41): 10357-10362.
Hart MR, et al. (2018) Activating pax gene family paralogs to complement pax5 leukemia driver mutations. PLoS Genet 14 (9): e1007642.