Human Runt Related Transcription Factor 3 (RUNX3) ELISA Kit | RUNX3 elisa kit

Human Runt Related Transcription Factor 3 (RUNX3) ELISA Kit

Cat. Num. AAA2705219

• Gene Names: RUNX3; AML2; CBFA3; PEBP2aC
• Reactivity: Human
• Synonyms: Runt Related Transcription Factor 3 (RUNX3); Human Runt Related Transcription Factor 3 (RUNX3) ELISA Kit; AML2; CBFA3; PEBP2aC; PEBP2A3; Acute myeloid leukemia 2 protein; Core-binding factor subunit alpha-3; Polyomavirus enhancer-binding protein 2 alpha C subunit; RUNX3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Runt Related Transcription Factor 3 (RUNX3).; No significant cross-reactivity or interference between Runt Related Transcription Factor 3 (RUNX3) and analogues was observed.
• Sequence Length: 429

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Runt Related Transcription Factor 3 (RUNX3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Runt Related Transcription Factor 3 (RUNX3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RUNX3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Runt Related Transcription Factor 3 (RUNX3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Runt Related Transcription Factor 3 (RUNX3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Runt Related Transcription Factor 3 (RUNX3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Runt Related Transcription Factor 3 (RUNX3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 72534652
• NCBI GeneID: 864
• NCBI Accession #: NP_001026850.1
• NCBI GenBank Nucleotide #: NM_001031680.2
• UniProt Accession #: Q13761; Q12969; Q13760; B1AJV5
• Molecular Weight: 45,922 Da
• NCBI Official Full Name: runt-related transcription factor 3 isoform 1
• NCBI Official Synonym Full Names: runt related transcription factor 3
• NCBI Official Symbol: RUNX3
• NCBI Official Synonym Symbols: AML2; CBFA3; PEBP2aC
• NCBI Protein Information: runt-related transcription factor 3
• UniProt Protein Name: Runt-related transcription factor 3
• Protein Family: Runt-related transcription factor
• UniProt Gene Name: RUNX3
• UniProt Synonym Gene Names: AML2; CBFA3; PEBP2A3; CBF-alpha-3; PEA2-alpha C; PEBP2-alpha C

NCBI Description

This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Uniprot Description

AML2: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters. Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA- binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1. The tyrosine phosphorylated form (via runt domain) interacts with SRC (via protein kinase domain). Interacts with FYN and LCK. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; nuclear chromatin; nucleolus; nucleoplasm; nucleus

Molecular Function: ATP binding; DNA binding transcription factor activity; protein binding

Biological Process: chondrocyte differentiation; hemopoiesis; negative regulation of cell cycle; negative regulation of epithelial cell proliferation; negative regulation of transcription from RNA polymerase II promoter; ossification; peripheral nervous system neuron development; positive regulation of transcription, DNA-templated; protein amino acid phosphorylation; regulation of cell differentiation; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; response to transforming growth factor beta; transcription from RNA polymerase II promoter